A behavioral and immunohistochemical study on the development of perirhinal cortical kindling: A comparison with other types of limbic kindling

Toshiki Sato, Norihito Yamada, Kiyoshi Morimoto, Shuji Uemura, Shigetoshi Kuroda

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We have previously reported that the perirhinal cortex (PRC) plays an important role in the generalization of kindled seizures. In the present study, we kindled the rat PRC and made a comparison with amygdala (AM) and dorsal hippocampal (dHIPP) kindling. In order to produce a functional map of the seizure generalization pathway from these limbic foci, we also stained for Fos protein in sections of the PRC-, AM- and dHIPP-kindled brains using an immunohistochemistry technique. In the generalized seizures of PRC kindling the duration of afterdischarges (ADs) and the latency to forelimb clonus were significantly shorter than those of AM kindling or dHIPP kindling. Typically, the PRC-kindled rats demonstrated moving arrest or exploratory behavior for about 10 days, and then a characteristic 'rapid backward moving' behavior for 1 day, followed by the sudden appearance of generalized motor seizures. Fos protein induction after a single stimulation of the PRC is more widely observed than after a single stimulation of the AM, in that the PRC stimulation produced Fos protein expression in the temporal and parietal neocortices. Following AM and PRC kindling, the Fos-positive areas were asymmetrically propagated from the ipsilateral to the contralateral hemisphere. The contralateral PRC was primarily activated at the generalization of epileptic activity in the contralateral hemisphere. In contrast, the Fos protein distribution of the dHIPP-kindled rats was restricted to the bilateral hippocampi during the early stages, followed by the symmetrical propagation from the limbic system to the neocortex during the generalized seizures. These results indicate that the PRC plays a characteristic role in the seizure generalization of kindling.

Original languageEnglish
Pages (from-to)122-132
Number of pages11
JournalBrain Research
Volume811
Issue number1-2
DOIs
Publication statusPublished - Nov 16 1998

Fingerprint

Seizures
Amygdala
Neocortex
Proteins
Perirhinal Cortex
Limbic System
Exploratory Behavior
Forelimb
Hippocampus
Immunohistochemistry
Brain

Keywords

  • Amygdala
  • Dorsal hippocampus
  • Epilepsy
  • Fos protein
  • Kindling
  • Perirhinal cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A behavioral and immunohistochemical study on the development of perirhinal cortical kindling : A comparison with other types of limbic kindling. / Sato, Toshiki; Yamada, Norihito; Morimoto, Kiyoshi; Uemura, Shuji; Kuroda, Shigetoshi.

In: Brain Research, Vol. 811, No. 1-2, 16.11.1998, p. 122-132.

Research output: Contribution to journalArticle

Sato, Toshiki ; Yamada, Norihito ; Morimoto, Kiyoshi ; Uemura, Shuji ; Kuroda, Shigetoshi. / A behavioral and immunohistochemical study on the development of perirhinal cortical kindling : A comparison with other types of limbic kindling. In: Brain Research. 1998 ; Vol. 811, No. 1-2. pp. 122-132.
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