A 50-kDa variant form of human surfactant protein D

R. J. Mason, L. D. Nielsen, Y. Kuroki, Eiji Matsuura, J. H. Freed, J. M. Shannon

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The dominant form of human surfactant protein D (SP-D) is a multimeric collagenous glycoprotein composed of monomeric subunits that have a molecular mass of 43 kDa under reducing conditions. However, in evaluating monoclonal antibodies to human SP-D, an additional monomeric subunit was identified with a reduced molecular mass of 50 kDa. This 50-kDa variant was detected in approximately half of the samples evaluated and was found in lavage fluid from normal subjects, patients with alveolar proteinosis or idiopathic pulmonary fibrosis and in amniotic fluid. This 50-kDa variant had the same amino-terminal sequence, amino acid composition and apparent size of the carboxy-terminal collagenase-resistant fragment (20 kDa) as the 43-kDa subunit. The major difference was in the amino-terminal portion of the molecule and was due to altered glycosylation, as determined by carbohydrate staining, chemical deglycosylation, treatment with N-glycanase and neuraminidase and reduced signals for threonine at positions 5, 9 and 10 during amino-terminal sequencing. After gel filtration chromatography, the 50-kDa form was not present in the high molecular weight fraction, which is commonly used in purification of SP-D, but was found only in the smaller molecular weight fraction of monomers and trimers of SP-D. In conclusion, the 50 kDa-form of surfactant protein D is produced by post-translational glycosylation and does not form higher ordered oligomers, but its precise physiological function remains to be determined.

Original languageEnglish
Pages (from-to)1147-1155
Number of pages9
JournalEuropean Respiratory Journal
Volume12
Issue number5
Publication statusPublished - Nov 1998

Fingerprint

Pulmonary Surfactant-Associated Protein D
Glycosylation
Molecular Weight
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Idiopathic Pulmonary Fibrosis
Therapeutic Irrigation
Neuraminidase
Collagenases
Threonine
Amniotic Fluid
Gel Chromatography
Amino Acid Sequence
Glycoproteins
Monoclonal Antibodies
Carbohydrates
Staining and Labeling

Keywords

  • Host defence
  • Pulmonary surface active material
  • Surfactant
  • Surfactant protein D

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Mason, R. J., Nielsen, L. D., Kuroki, Y., Matsuura, E., Freed, J. H., & Shannon, J. M. (1998). A 50-kDa variant form of human surfactant protein D. European Respiratory Journal, 12(5), 1147-1155.

A 50-kDa variant form of human surfactant protein D. / Mason, R. J.; Nielsen, L. D.; Kuroki, Y.; Matsuura, Eiji; Freed, J. H.; Shannon, J. M.

In: European Respiratory Journal, Vol. 12, No. 5, 11.1998, p. 1147-1155.

Research output: Contribution to journalArticle

Mason, RJ, Nielsen, LD, Kuroki, Y, Matsuura, E, Freed, JH & Shannon, JM 1998, 'A 50-kDa variant form of human surfactant protein D', European Respiratory Journal, vol. 12, no. 5, pp. 1147-1155.
Mason RJ, Nielsen LD, Kuroki Y, Matsuura E, Freed JH, Shannon JM. A 50-kDa variant form of human surfactant protein D. European Respiratory Journal. 1998 Nov;12(5):1147-1155.
Mason, R. J. ; Nielsen, L. D. ; Kuroki, Y. ; Matsuura, Eiji ; Freed, J. H. ; Shannon, J. M. / A 50-kDa variant form of human surfactant protein D. In: European Respiratory Journal. 1998 ; Vol. 12, No. 5. pp. 1147-1155.
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