8Th iwa symposium on waste management problems in agro-industries- agro'2011: Comparison of simultaneous and separate processes: Saccharification and thermophilic L-lactate fermentation of catch crop and aquatic plant biomass

Satoshi Akao, Koutaro Maeda, Shingo Nakatani, Yoshihiko Hosoi, Hideaki Nagare, Morihiro Maeda, Taku Fujiwara

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Catch crop candidates (corn, guinea grass) for recovering nutrients from farm soil and aquatic plants (water caltrop, water hyacinth) were utilized to produce l-lactic acid. The efficiencies of pre-treatment methods for enzymatic saccharification and l-lactate production of two fermentation processes, thermophilic simultaneous saccharification and fermentation (SSF), as well as separate saccharification and fermentation, were compared. Conditions were set at 55°C and pH 5.5 for non-sterile fermentation. Alkaline/peroxide pre-treatment proved the most effective for saccharification in pre-treated corn, guinea grass, water caltrop and water hyacinth with glucose yields of 0.23, 0.20, 0.11 and 0.14 g/g-dry native biomass (24-hour incubation period), respectively. Examination of the two types of thermophilic l-lactate fermentation employed following alkaline/peroxide pre-treatment and saccharification demonstrated that the l-lactate yield obtained using SSF (0.15 g/g in the case of corn) was lower than that obtained using separate saccharification and fermentation (0.28 g/g in the case of corn). The lower yield obtained from SSF is likely to have resulted from the saccharification conditions used in the present study, as the possibility of cellulase deactivation during SSF by thermophilic l-lactate producing bacteria existed. A cellulase that retains high activity levels under non-sterile conditions and a l-lactate producer without cellulose hydrolysis activity would be required in order for SSF to serve as an effective method of l-lactate production.

Original languageEnglish
Pages (from-to)1523-1529
Number of pages7
JournalEnvironmental Technology
Volume33
Issue number13
DOIs
Publication statusPublished - 2012

Fingerprint

catch crop
Saccharification
crop plant
aquatic plant
Waste management
Fermentation
Crops
fermentation
waste management
Lactic Acid
Biomass
industry
biomass
Industry
maize
Cellulase
Water
Peroxides
comparison
grass

Keywords

  • Alkaline/peroxide
  • Bacillus coagulans
  • Cellulase deactivation
  • Pre-treatment
  • SSF

ASJC Scopus subject areas

  • Environmental Chemistry
  • Waste Management and Disposal
  • Water Science and Technology

Cite this

@article{5f8567cc2821468e91a4afe43c345802,
title = "8Th iwa symposium on waste management problems in agro-industries- agro'2011: Comparison of simultaneous and separate processes: Saccharification and thermophilic L-lactate fermentation of catch crop and aquatic plant biomass",
abstract = "Catch crop candidates (corn, guinea grass) for recovering nutrients from farm soil and aquatic plants (water caltrop, water hyacinth) were utilized to produce l-lactic acid. The efficiencies of pre-treatment methods for enzymatic saccharification and l-lactate production of two fermentation processes, thermophilic simultaneous saccharification and fermentation (SSF), as well as separate saccharification and fermentation, were compared. Conditions were set at 55°C and pH 5.5 for non-sterile fermentation. Alkaline/peroxide pre-treatment proved the most effective for saccharification in pre-treated corn, guinea grass, water caltrop and water hyacinth with glucose yields of 0.23, 0.20, 0.11 and 0.14 g/g-dry native biomass (24-hour incubation period), respectively. Examination of the two types of thermophilic l-lactate fermentation employed following alkaline/peroxide pre-treatment and saccharification demonstrated that the l-lactate yield obtained using SSF (0.15 g/g in the case of corn) was lower than that obtained using separate saccharification and fermentation (0.28 g/g in the case of corn). The lower yield obtained from SSF is likely to have resulted from the saccharification conditions used in the present study, as the possibility of cellulase deactivation during SSF by thermophilic l-lactate producing bacteria existed. A cellulase that retains high activity levels under non-sterile conditions and a l-lactate producer without cellulose hydrolysis activity would be required in order for SSF to serve as an effective method of l-lactate production.",
keywords = "Alkaline/peroxide, Bacillus coagulans, Cellulase deactivation, Pre-treatment, SSF",
author = "Satoshi Akao and Koutaro Maeda and Shingo Nakatani and Yoshihiko Hosoi and Hideaki Nagare and Morihiro Maeda and Taku Fujiwara",
year = "2012",
doi = "10.1080/09593330.2012.669412",
language = "English",
volume = "33",
pages = "1523--1529",
journal = "Environmental Technology (United Kingdom)",
issn = "0959-3330",
publisher = "Taylor and Francis Ltd.",
number = "13",

}

TY - JOUR

T1 - 8Th iwa symposium on waste management problems in agro-industries- agro'2011

T2 - Comparison of simultaneous and separate processes: Saccharification and thermophilic L-lactate fermentation of catch crop and aquatic plant biomass

AU - Akao, Satoshi

AU - Maeda, Koutaro

AU - Nakatani, Shingo

AU - Hosoi, Yoshihiko

AU - Nagare, Hideaki

AU - Maeda, Morihiro

AU - Fujiwara, Taku

PY - 2012

Y1 - 2012

N2 - Catch crop candidates (corn, guinea grass) for recovering nutrients from farm soil and aquatic plants (water caltrop, water hyacinth) were utilized to produce l-lactic acid. The efficiencies of pre-treatment methods for enzymatic saccharification and l-lactate production of two fermentation processes, thermophilic simultaneous saccharification and fermentation (SSF), as well as separate saccharification and fermentation, were compared. Conditions were set at 55°C and pH 5.5 for non-sterile fermentation. Alkaline/peroxide pre-treatment proved the most effective for saccharification in pre-treated corn, guinea grass, water caltrop and water hyacinth with glucose yields of 0.23, 0.20, 0.11 and 0.14 g/g-dry native biomass (24-hour incubation period), respectively. Examination of the two types of thermophilic l-lactate fermentation employed following alkaline/peroxide pre-treatment and saccharification demonstrated that the l-lactate yield obtained using SSF (0.15 g/g in the case of corn) was lower than that obtained using separate saccharification and fermentation (0.28 g/g in the case of corn). The lower yield obtained from SSF is likely to have resulted from the saccharification conditions used in the present study, as the possibility of cellulase deactivation during SSF by thermophilic l-lactate producing bacteria existed. A cellulase that retains high activity levels under non-sterile conditions and a l-lactate producer without cellulose hydrolysis activity would be required in order for SSF to serve as an effective method of l-lactate production.

AB - Catch crop candidates (corn, guinea grass) for recovering nutrients from farm soil and aquatic plants (water caltrop, water hyacinth) were utilized to produce l-lactic acid. The efficiencies of pre-treatment methods for enzymatic saccharification and l-lactate production of two fermentation processes, thermophilic simultaneous saccharification and fermentation (SSF), as well as separate saccharification and fermentation, were compared. Conditions were set at 55°C and pH 5.5 for non-sterile fermentation. Alkaline/peroxide pre-treatment proved the most effective for saccharification in pre-treated corn, guinea grass, water caltrop and water hyacinth with glucose yields of 0.23, 0.20, 0.11 and 0.14 g/g-dry native biomass (24-hour incubation period), respectively. Examination of the two types of thermophilic l-lactate fermentation employed following alkaline/peroxide pre-treatment and saccharification demonstrated that the l-lactate yield obtained using SSF (0.15 g/g in the case of corn) was lower than that obtained using separate saccharification and fermentation (0.28 g/g in the case of corn). The lower yield obtained from SSF is likely to have resulted from the saccharification conditions used in the present study, as the possibility of cellulase deactivation during SSF by thermophilic l-lactate producing bacteria existed. A cellulase that retains high activity levels under non-sterile conditions and a l-lactate producer without cellulose hydrolysis activity would be required in order for SSF to serve as an effective method of l-lactate production.

KW - Alkaline/peroxide

KW - Bacillus coagulans

KW - Cellulase deactivation

KW - Pre-treatment

KW - SSF

UR - http://www.scopus.com/inward/record.url?scp=84864303704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864303704&partnerID=8YFLogxK

U2 - 10.1080/09593330.2012.669412

DO - 10.1080/09593330.2012.669412

M3 - Article

C2 - 22988611

AN - SCOPUS:84864303704

VL - 33

SP - 1523

EP - 1529

JO - Environmental Technology (United Kingdom)

JF - Environmental Technology (United Kingdom)

SN - 0959-3330

IS - 13

ER -