Abstract
Aims: 7-Ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxidized low-density lipoprotein (oxLDL), has been reported to be a crucial ligand of beta2-glycoprotein I (β2-GPI), and plays a potential role in the development of atherosclerosis (AS), however, the role of the sole oxLig-1 in the development of AS remains unclear. Main methods: Expression and phosphorylation levels of several proteins, such as nuclear factor-kappaB (NF-κB), protein kinase C (PKC), IκBβ and inter-cellular adhesion molecule 1 (ICAM-1) were determined by Western blot; nuclear localization of NF-κB was studied by immunocytochemistry; NF-κB activation was assayed by electrophoretic mobility shift assay (EMSA); and expressions of genes associated with AS process were investigated by Mouse Atherosclerosis RT2 Profiler PCR Array assay. Key findings: The present work indicated that oxLig-1 induced IκBβ phosphorylation and results in the nuclear translocation of NF-κB in J774A.1 macrophages. Moreover, oxLig-1-induced NF-κB DNA binding activity was detected by EMSA. Indeed, oxLig-1 led to the activation of PKC prior to activating NF-κB. The treatment of oxLig-1 in J774A.1 macrophages up-regulates the expression of NF-κB target genes including ICAM-1. Significance: OxLig-1 on the oxLDL plays an important role in AS process, as evidenced by the NF-κB activation and up-regulation of genes involved in AS development in oxLig-1 challenged J774A.1 macrophages.
| Original language | English |
|---|---|
| Pages (from-to) | 651-657 |
| Number of pages | 7 |
| Journal | Life Sciences |
| Volume | 87 |
| Issue number | 19-22 |
| DOIs | |
| Publication status | Published - Nov 20 2010 |
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Keywords
- ICAM-1
- Macrophage
- NF-κB
- OxLig-1
- PKC
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
7-Ketocholesteryl-9-carboxynonanoate induced nuclear factor-kappa B activation in J774A.1 macrophages. / Huang, Zhenyu; Li, Wenzhe; Wang, Renjun; Zhang, Fan; Chi, Yan; Wang, Dan; Liu, Zhe; Zhang, Yingbiao; Matsuura, Eiji; Liu, Qingping.
In: Life Sciences, Vol. 87, No. 19-22, 20.11.2010, p. 651-657.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 7-Ketocholesteryl-9-carboxynonanoate induced nuclear factor-kappa B activation in J774A.1 macrophages
AU - Huang, Zhenyu
AU - Li, Wenzhe
AU - Wang, Renjun
AU - Zhang, Fan
AU - Chi, Yan
AU - Wang, Dan
AU - Liu, Zhe
AU - Zhang, Yingbiao
AU - Matsuura, Eiji
AU - Liu, Qingping
PY - 2010/11/20
Y1 - 2010/11/20
N2 - Aims: 7-Ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxidized low-density lipoprotein (oxLDL), has been reported to be a crucial ligand of beta2-glycoprotein I (β2-GPI), and plays a potential role in the development of atherosclerosis (AS), however, the role of the sole oxLig-1 in the development of AS remains unclear. Main methods: Expression and phosphorylation levels of several proteins, such as nuclear factor-kappaB (NF-κB), protein kinase C (PKC), IκBβ and inter-cellular adhesion molecule 1 (ICAM-1) were determined by Western blot; nuclear localization of NF-κB was studied by immunocytochemistry; NF-κB activation was assayed by electrophoretic mobility shift assay (EMSA); and expressions of genes associated with AS process were investigated by Mouse Atherosclerosis RT2 Profiler PCR Array assay. Key findings: The present work indicated that oxLig-1 induced IκBβ phosphorylation and results in the nuclear translocation of NF-κB in J774A.1 macrophages. Moreover, oxLig-1-induced NF-κB DNA binding activity was detected by EMSA. Indeed, oxLig-1 led to the activation of PKC prior to activating NF-κB. The treatment of oxLig-1 in J774A.1 macrophages up-regulates the expression of NF-κB target genes including ICAM-1. Significance: OxLig-1 on the oxLDL plays an important role in AS process, as evidenced by the NF-κB activation and up-regulation of genes involved in AS development in oxLig-1 challenged J774A.1 macrophages.
AB - Aims: 7-Ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxidized low-density lipoprotein (oxLDL), has been reported to be a crucial ligand of beta2-glycoprotein I (β2-GPI), and plays a potential role in the development of atherosclerosis (AS), however, the role of the sole oxLig-1 in the development of AS remains unclear. Main methods: Expression and phosphorylation levels of several proteins, such as nuclear factor-kappaB (NF-κB), protein kinase C (PKC), IκBβ and inter-cellular adhesion molecule 1 (ICAM-1) were determined by Western blot; nuclear localization of NF-κB was studied by immunocytochemistry; NF-κB activation was assayed by electrophoretic mobility shift assay (EMSA); and expressions of genes associated with AS process were investigated by Mouse Atherosclerosis RT2 Profiler PCR Array assay. Key findings: The present work indicated that oxLig-1 induced IκBβ phosphorylation and results in the nuclear translocation of NF-κB in J774A.1 macrophages. Moreover, oxLig-1-induced NF-κB DNA binding activity was detected by EMSA. Indeed, oxLig-1 led to the activation of PKC prior to activating NF-κB. The treatment of oxLig-1 in J774A.1 macrophages up-regulates the expression of NF-κB target genes including ICAM-1. Significance: OxLig-1 on the oxLDL plays an important role in AS process, as evidenced by the NF-κB activation and up-regulation of genes involved in AS development in oxLig-1 challenged J774A.1 macrophages.
KW - ICAM-1
KW - Macrophage
KW - NF-κB
KW - OxLig-1
KW - PKC
UR - http://www.scopus.com/inward/record.url?scp=78049448735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78049448735&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2010.09.028
DO - 10.1016/j.lfs.2010.09.028
M3 - Article
C2 - 20932850
AN - SCOPUS:78049448735
VL - 87
SP - 651
EP - 657
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 19-22
ER -