7-Ketocholesteryl-9-carboxynonanoate induced nuclear factor-kappa B activation in J774A.1 macrophages

Zhenyu Huang, Wenzhe Li, Renjun Wang, Fan Zhang, Yan Chi, Dan Wang, Zhe Liu, Yingbiao Zhang, Eiji Matsuura, Qingping Liu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aims: 7-Ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxidized low-density lipoprotein (oxLDL), has been reported to be a crucial ligand of beta2-glycoprotein I (β2-GPI), and plays a potential role in the development of atherosclerosis (AS), however, the role of the sole oxLig-1 in the development of AS remains unclear. Main methods: Expression and phosphorylation levels of several proteins, such as nuclear factor-kappaB (NF-κB), protein kinase C (PKC), IκBβ and inter-cellular adhesion molecule 1 (ICAM-1) were determined by Western blot; nuclear localization of NF-κB was studied by immunocytochemistry; NF-κB activation was assayed by electrophoretic mobility shift assay (EMSA); and expressions of genes associated with AS process were investigated by Mouse Atherosclerosis RT2 Profiler PCR Array assay. Key findings: The present work indicated that oxLig-1 induced IκBβ phosphorylation and results in the nuclear translocation of NF-κB in J774A.1 macrophages. Moreover, oxLig-1-induced NF-κB DNA binding activity was detected by EMSA. Indeed, oxLig-1 led to the activation of PKC prior to activating NF-κB. The treatment of oxLig-1 in J774A.1 macrophages up-regulates the expression of NF-κB target genes including ICAM-1. Significance: OxLig-1 on the oxLDL plays an important role in AS process, as evidenced by the NF-κB activation and up-regulation of genes involved in AS development in oxLig-1 challenged J774A.1 macrophages.

Original languageEnglish
Pages (from-to)651-657
Number of pages7
JournalLife Sciences
Volume87
Issue number19-22
DOIs
Publication statusPublished - Nov 20 2010

Keywords

  • ICAM-1
  • Macrophage
  • NF-κB
  • OxLig-1
  • PKC

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Huang, Z., Li, W., Wang, R., Zhang, F., Chi, Y., Wang, D., Liu, Z., Zhang, Y., Matsuura, E., & Liu, Q. (2010). 7-Ketocholesteryl-9-carboxynonanoate induced nuclear factor-kappa B activation in J774A.1 macrophages. Life Sciences, 87(19-22), 651-657. https://doi.org/10.1016/j.lfs.2010.09.028