7-Ketocholesteryl-9-carboxynonanoate induced nuclear factor-kappa B activation in J774A.1 macrophages

Zhenyu Huang, Wenzhe Li, Renjun Wang, Fan Zhang, Yan Chi, Dan Wang, Zhe Liu, Yingbiao Zhang, Eiji Matsuura, Qingping Liu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aims: 7-Ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxidized low-density lipoprotein (oxLDL), has been reported to be a crucial ligand of beta2-glycoprotein I (β2-GPI), and plays a potential role in the development of atherosclerosis (AS), however, the role of the sole oxLig-1 in the development of AS remains unclear. Main methods: Expression and phosphorylation levels of several proteins, such as nuclear factor-kappaB (NF-κB), protein kinase C (PKC), IκBβ and inter-cellular adhesion molecule 1 (ICAM-1) were determined by Western blot; nuclear localization of NF-κB was studied by immunocytochemistry; NF-κB activation was assayed by electrophoretic mobility shift assay (EMSA); and expressions of genes associated with AS process were investigated by Mouse Atherosclerosis RT2 Profiler PCR Array assay. Key findings: The present work indicated that oxLig-1 induced IκBβ phosphorylation and results in the nuclear translocation of NF-κB in J774A.1 macrophages. Moreover, oxLig-1-induced NF-κB DNA binding activity was detected by EMSA. Indeed, oxLig-1 led to the activation of PKC prior to activating NF-κB. The treatment of oxLig-1 in J774A.1 macrophages up-regulates the expression of NF-κB target genes including ICAM-1. Significance: OxLig-1 on the oxLDL plays an important role in AS process, as evidenced by the NF-κB activation and up-regulation of genes involved in AS development in oxLig-1 challenged J774A.1 macrophages.

Original languageEnglish
Pages (from-to)651-657
Number of pages7
JournalLife Sciences
Volume87
Issue number19-22
DOIs
Publication statusPublished - Nov 20 2010

Fingerprint

NF-kappa B
Macrophages
Chemical activation
Atherosclerosis
Assays
Electrophoretic mobility
Phosphorylation
Genes
Electrophoretic Mobility Shift Assay
Protein Kinase C
Up-Regulation
Adhesion
beta 2-Glycoprotein I
7-ketocholesteryl-9-carboxynonanoate
Molecules
Western Blotting
Immunohistochemistry
Ligands
Lipids
Gene Expression

Keywords

  • ICAM-1
  • Macrophage
  • NF-κB
  • OxLig-1
  • PKC

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

7-Ketocholesteryl-9-carboxynonanoate induced nuclear factor-kappa B activation in J774A.1 macrophages. / Huang, Zhenyu; Li, Wenzhe; Wang, Renjun; Zhang, Fan; Chi, Yan; Wang, Dan; Liu, Zhe; Zhang, Yingbiao; Matsuura, Eiji; Liu, Qingping.

In: Life Sciences, Vol. 87, No. 19-22, 20.11.2010, p. 651-657.

Research output: Contribution to journalArticle

Huang, Z, Li, W, Wang, R, Zhang, F, Chi, Y, Wang, D, Liu, Z, Zhang, Y, Matsuura, E & Liu, Q 2010, '7-Ketocholesteryl-9-carboxynonanoate induced nuclear factor-kappa B activation in J774A.1 macrophages', Life Sciences, vol. 87, no. 19-22, pp. 651-657. https://doi.org/10.1016/j.lfs.2010.09.028
Huang, Zhenyu ; Li, Wenzhe ; Wang, Renjun ; Zhang, Fan ; Chi, Yan ; Wang, Dan ; Liu, Zhe ; Zhang, Yingbiao ; Matsuura, Eiji ; Liu, Qingping. / 7-Ketocholesteryl-9-carboxynonanoate induced nuclear factor-kappa B activation in J774A.1 macrophages. In: Life Sciences. 2010 ; Vol. 87, No. 19-22. pp. 651-657.
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AU - Huang, Zhenyu

AU - Li, Wenzhe

AU - Wang, Renjun

AU - Zhang, Fan

AU - Chi, Yan

AU - Wang, Dan

AU - Liu, Zhe

AU - Zhang, Yingbiao

AU - Matsuura, Eiji

AU - Liu, Qingping

PY - 2010/11/20

Y1 - 2010/11/20

N2 - Aims: 7-Ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxidized low-density lipoprotein (oxLDL), has been reported to be a crucial ligand of beta2-glycoprotein I (β2-GPI), and plays a potential role in the development of atherosclerosis (AS), however, the role of the sole oxLig-1 in the development of AS remains unclear. Main methods: Expression and phosphorylation levels of several proteins, such as nuclear factor-kappaB (NF-κB), protein kinase C (PKC), IκBβ and inter-cellular adhesion molecule 1 (ICAM-1) were determined by Western blot; nuclear localization of NF-κB was studied by immunocytochemistry; NF-κB activation was assayed by electrophoretic mobility shift assay (EMSA); and expressions of genes associated with AS process were investigated by Mouse Atherosclerosis RT2 Profiler PCR Array assay. Key findings: The present work indicated that oxLig-1 induced IκBβ phosphorylation and results in the nuclear translocation of NF-κB in J774A.1 macrophages. Moreover, oxLig-1-induced NF-κB DNA binding activity was detected by EMSA. Indeed, oxLig-1 led to the activation of PKC prior to activating NF-κB. The treatment of oxLig-1 in J774A.1 macrophages up-regulates the expression of NF-κB target genes including ICAM-1. Significance: OxLig-1 on the oxLDL plays an important role in AS process, as evidenced by the NF-κB activation and up-regulation of genes involved in AS development in oxLig-1 challenged J774A.1 macrophages.

AB - Aims: 7-Ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxidized low-density lipoprotein (oxLDL), has been reported to be a crucial ligand of beta2-glycoprotein I (β2-GPI), and plays a potential role in the development of atherosclerosis (AS), however, the role of the sole oxLig-1 in the development of AS remains unclear. Main methods: Expression and phosphorylation levels of several proteins, such as nuclear factor-kappaB (NF-κB), protein kinase C (PKC), IκBβ and inter-cellular adhesion molecule 1 (ICAM-1) were determined by Western blot; nuclear localization of NF-κB was studied by immunocytochemistry; NF-κB activation was assayed by electrophoretic mobility shift assay (EMSA); and expressions of genes associated with AS process were investigated by Mouse Atherosclerosis RT2 Profiler PCR Array assay. Key findings: The present work indicated that oxLig-1 induced IκBβ phosphorylation and results in the nuclear translocation of NF-κB in J774A.1 macrophages. Moreover, oxLig-1-induced NF-κB DNA binding activity was detected by EMSA. Indeed, oxLig-1 led to the activation of PKC prior to activating NF-κB. The treatment of oxLig-1 in J774A.1 macrophages up-regulates the expression of NF-κB target genes including ICAM-1. Significance: OxLig-1 on the oxLDL plays an important role in AS process, as evidenced by the NF-κB activation and up-regulation of genes involved in AS development in oxLig-1 challenged J774A.1 macrophages.

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KW - NF-κB

KW - OxLig-1

KW - PKC

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