7-Ketocholesterol induces cell apoptosis by activation of nuclear factor kappa B in mouse macrophages

Zhenyu Huang, Qingping Liu, Wenzhe Li, Renjun Wang, Dan Wang, Yingbiao Zhang, Fan Zhang, Yan Chi, Zhe Liu, Eiji Matsuura, Zibo Liuc, Qiming Zhang

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse mono-cytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-κB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-κB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-κB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF/cB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-κB.

    Original languageEnglish
    Pages (from-to)85-93
    Number of pages9
    JournalActa medica Okayama
    Volume64
    Issue number2
    Publication statusPublished - Apr 2010

    Keywords

    • 7-KC
    • Apoptosis
    • Atherosclerosis
    • NF-κB

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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