7-Ketocholesterol induces cell apoptosis by activation of nuclear factor kappa B in mouse macrophages

Zhenyu Huang, Qingping Liu, Wenzhe Li, Renjun Wang, Dan Wang, Yingbiao Zhang, Fan Zhang, Yan Chi, Zhe Liu, Eiji Matsuura, Zibo Liuc, Qiming Zhang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse mono-cytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-κB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-κB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-κB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF/cB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-κB.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalActa Medica Okayama
Volume64
Issue number2
Publication statusPublished - Apr 2010

Fingerprint

NF-kappa B
Macrophages
Chemical activation
Apoptosis
Cells
Electrophoretic mobility
Propidium
Annexin A5
DNA
Electrophoretic Mobility Shift Assay
Assays
Atherosclerosis
Binding Sites
7-ketocholesterol
Staining and Labeling
Cell Line

Keywords

  • 7-KC
  • Apoptosis
  • Atherosclerosis
  • NF-κB

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Huang, Z., Liu, Q., Li, W., Wang, R., Wang, D., Zhang, Y., ... Zhang, Q. (2010). 7-Ketocholesterol induces cell apoptosis by activation of nuclear factor kappa B in mouse macrophages. Acta Medica Okayama, 64(2), 85-93.

7-Ketocholesterol induces cell apoptosis by activation of nuclear factor kappa B in mouse macrophages. / Huang, Zhenyu; Liu, Qingping; Li, Wenzhe; Wang, Renjun; Wang, Dan; Zhang, Yingbiao; Zhang, Fan; Chi, Yan; Liu, Zhe; Matsuura, Eiji; Liuc, Zibo; Zhang, Qiming.

In: Acta Medica Okayama, Vol. 64, No. 2, 04.2010, p. 85-93.

Research output: Contribution to journalArticle

Huang, Z, Liu, Q, Li, W, Wang, R, Wang, D, Zhang, Y, Zhang, F, Chi, Y, Liu, Z, Matsuura, E, Liuc, Z & Zhang, Q 2010, '7-Ketocholesterol induces cell apoptosis by activation of nuclear factor kappa B in mouse macrophages', Acta Medica Okayama, vol. 64, no. 2, pp. 85-93.
Huang, Zhenyu ; Liu, Qingping ; Li, Wenzhe ; Wang, Renjun ; Wang, Dan ; Zhang, Yingbiao ; Zhang, Fan ; Chi, Yan ; Liu, Zhe ; Matsuura, Eiji ; Liuc, Zibo ; Zhang, Qiming. / 7-Ketocholesterol induces cell apoptosis by activation of nuclear factor kappa B in mouse macrophages. In: Acta Medica Okayama. 2010 ; Vol. 64, No. 2. pp. 85-93.
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