3-dimensional tissue is formed from cancer cells in vitro on gelfoam ®, but not on matrigelTM

Yasunori Tome, Fuminari Uehara, Sumiyuki Mii, Shuuya Yano, Lei Zhang, Naotoshi Sugimoto, Hiroki Maehara, Michael Bouvet, Hiroyuki Tsuchiya, Fuminori Kanaya, Robert M. Hoffman

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23 Citations (Scopus)

Abstract

Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam®, a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to DNA synthesis and cell growth. Therefore, the flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of αv integrin and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel™, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam®, the cells formed three-dimensional tissue-like structures. The behavior of 143B osteosarcoma cells on Gelfoam ® in culture is remarkably different from those of these cells in monolayer culture or in Matrigel™. Tissue-like structures were observed only in Gelfoam® culture. The data in this report suggest a flexible structural substrate such as Gelfoam® provides a more in vivo-like culture condition than monolayer culture or MatrigelTM and that MatrigelTM does not result in actual three-dimensional culture. J. Cell. Biochem. 115: 1362-1367, 2014.

Original languageEnglish
Pages (from-to)1362-1367
Number of pages6
JournalJournal of Cellular Biochemistry
Volume115
Issue number8
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

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Keywords

  • confocal microscopy
  • culture
  • dimension
  • green fluorescent protein
  • histology
  • matrigel™
  • osteosarcoma
  • real-time imaging

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tome, Y., Uehara, F., Mii, S., Yano, S., Zhang, L., Sugimoto, N., Maehara, H., Bouvet, M., Tsuchiya, H., Kanaya, F., & Hoffman, R. M. (2014). 3-dimensional tissue is formed from cancer cells in vitro on gelfoam ®, but not on matrigelTM. Journal of Cellular Biochemistry, 115(8), 1362-1367. https://doi.org/10.1002/jcb.24780