Abstract
Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam®, a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to DNA synthesis and cell growth. Therefore, the flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of αv integrin and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel™, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam®, the cells formed three-dimensional tissue-like structures. The behavior of 143B osteosarcoma cells on Gelfoam ® in culture is remarkably different from those of these cells in monolayer culture or in Matrigel™. Tissue-like structures were observed only in Gelfoam® culture. The data in this report suggest a flexible structural substrate such as Gelfoam® provides a more in vivo-like culture condition than monolayer culture or MatrigelTM and that MatrigelTM does not result in actual three-dimensional culture. J. Cell. Biochem. 115: 1362-1367, 2014.
Original language | English |
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Pages (from-to) | 1362-1367 |
Number of pages | 6 |
Journal | Journal of Cellular Biochemistry |
Volume | 115 |
Issue number | 8 |
DOIs | |
Publication status | Published - Aug 2014 |
Externally published | Yes |
Keywords
- confocal microscopy
- culture
- dimension
- green fluorescent protein
- histology
- matrigel™
- osteosarcoma
- real-time imaging
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology