2,3-Butanedione monoxime suppresses primarily total calcium handling in canine heart

M. Maesako, J. Araki, S. Lee, Y. Doi, T. Imaoka, G. Iribe, S. Mohri, M. Hirakawa, M. Harada, H. Suga

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12 Citations (Scopus)

Abstract

Whether 2,3-butanedione monoxime (BDM, ≤5mmol/l) suppresses primarily crossbridge cycling or total Ca2+ handling in the blood-perfused whole heart remains controversial. Although BDM seems to suppress primarily total Ca2+ handling in canine hearts, more evidence is lacking. We therefore analyzed the cardiac mechanoenergetics, namely, Emax (contractility), PVA (total mechanical energy), and O2 consumption of canine BDM-treated hearts by our recently developed integrative method to assess myocardial total Ca2+ handling. This method additionally required the internal Ca2+ recirculation fraction. We obtained this from the beat constant of the exponential decay component of the postextrasystolic potentiation. Our analysis indicated significant decreases in both internal Ca2+ recirculation fraction and total Ca2+ handling in the BDM-treated heart, but virtually no change in the reactivity of Emax to total Ca2+ handling. This result corroborates the view that BDM suppresses primarily total Ca2+ handling rather than crossbridge cycling in the canine blood-perfused heart.

Original languageEnglish
Pages (from-to)543-551
Number of pages9
JournalJapanese journal of physiology
Volume50
Issue number5
DOIs
Publication statusPublished - Dec 1 2000

Keywords

  • Arrhythmia
  • Calcium
  • Contractility
  • Pressure
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Physiology

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    Maesako, M., Araki, J., Lee, S., Doi, Y., Imaoka, T., Iribe, G., Mohri, S., Hirakawa, M., Harada, M., & Suga, H. (2000). 2,3-Butanedione monoxime suppresses primarily total calcium handling in canine heart. Japanese journal of physiology, 50(5), 543-551. https://doi.org/10.2170/jjphysiol.50.543