Leaky coaxial cables (LCX) have been widely used as antennas to form linear service areas for radio communication systems. Such services of linear cells have been deployed inside tunnels, along railways, and so on. Usually, one LCX is utilized as one antenna. Therefore it requires more than one LCX to configure an multi-input multi-output (MIMO) system. In this paper, we propose a MIMO system using single LCX, which can be utilized as two antennas. When different RF transmit signals are fed to each end of the proposed cable, single LCX can work as two antennas. To confirm the feasibility of the proposed MIMO system, we conduct experiments to measure the characteristics of 2 × 2 MIMO channel using single LCX. The results confirm that our proposed 2×2 MIMO channel using single LCX can realize a good channel condition for MIMO transmission even within a highly correlated propagation condition. The proposed MIMO system using LCX will release the space requirement and reduce the cost of system for configuration of LCX MIMO system especially with large number of LCXs. It benefits the design and configuration of LCX-MIMO system for many scenarios of linear-cell.