1’prime;-Acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin

Akira Murakami, Shin Ohura, Yoshimasa Nakamura, Koichi Koshimizu, Hajime Ohigashi

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

The anti-tumor-promoting activity of 1’-acetoxychavicol acetate (ACA) was examined in a two-stage carcinogenesis experiment in ICR mouse skin using 7,12-dimethylbenz[a]anthracene (0.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA; 1.6 nmol). Topical application of ACA (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice: inhibition ratios 90% (p < 0.001) and 42% (p < 0.005), respectively. ACA even at a dose equimolar to TPA (1.6 nmol) significantly reduced theaverage number of tumors per mouse: inhibitory ratio 44% (p < 0.05). ACA potently inhibited TPA-induced superoxide (O2) generation in differentiated HL-60 cells (IC50 = 4.3μM) and suppressed the lipid hydroperoxide formation by42% (p < 0.001) in the ethyl linoleate autoxidation test.

Original languageEnglish
Pages (from-to)386-391
Number of pages6
JournalOncology (Switzerland)
Volume53
Issue number5
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

Keywords

  • 1’prime;-Acetoxychavicol acetate
  • Antioxidant
  • Antitumor promoter
  • Cancer chemoprevention
  • HL-60 cells
  • Languas galanga
  • Mouse skin
  • Superoxide anion
  • Xanthine oxidase

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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