1H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity

Nwe Haymar Lynn, Thein Zaw Linn, Cui Yanmei, Yuuki Shimozu, Shoko Taniguchi, Tsutomu Hatano

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We used quantitative nuclear magnetic resonance analyses to measure the contents of major constituents of Acorus rhizome materials used as herbal drugs. The inhibitory effects of crude n-hexane extracts and their individual constituents on in vitro acetylcholine esterase activity were evaluated. The crude extracts had unexpectedly weak inhibitory effects (46-64% inhibition at 1.0 mg/mL), despite the high content (46-64%) of β-asarone, which independently had a potent effect (IC50 2.9 µM [0.61 µg/mL]). Further investigation revealed participation of eudesmin A, a lignan constituent, in the suppression of the inhibitory effect of β-asarone.

Original languageEnglish
Pages (from-to)892-900
Number of pages9
JournalBioscience, biotechnology, and biochemistry
Volume83
Issue number5
DOIs
Publication statusPublished - May 1 2019

Keywords

  • acetylcholine esterase inhibitory activity
  • eudesmin A
  • qNMR
  • β-asarone

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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