1H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity

Nwe Haymar Lynn, Thein Zaw Linn, Cui Yanmei, Yuuki Shimozu, Shoko Taniguchi, Tsutomu Hatano

Research output: Contribution to journalArticle

Abstract

We used quantitative nuclear magnetic resonance analyses to measure the contents of major constituents of Acorus rhizome materials used as herbal drugs. The inhibitory effects of crude n-hexane extracts and their individual constituents on in vitro acetylcholine esterase activity were evaluated. The crude extracts had unexpectedly weak inhibitory effects (46-64% inhibition at 1.0 mg/mL), despite the high content (46-64%) of β-asarone, which independently had a potent effect (IC50 2.9 µM [0.61 µg/mL]). Further investigation revealed participation of eudesmin A, a lignan constituent, in the suppression of the inhibitory effect of β-asarone.

Original languageEnglish
Pages (from-to)892-900
Number of pages9
JournalBioscience, biotechnology, and biochemistry
Volume83
Issue number5
DOIs
Publication statusPublished - May 1 2019

Fingerprint

Acorus
Rhizome
Esterases
Quality Control
Acetylcholine
Quality control
Nuclear magnetic resonance
Lignans
Complex Mixtures
Pharmaceutical Preparations
Inhibitory Concentration 50
Magnetic Resonance Spectroscopy
In Vitro Techniques
Proton Magnetic Resonance Spectroscopy
asarone

Keywords

  • acetylcholine esterase inhibitory activity
  • eudesmin A
  • qNMR
  • β-asarone

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

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title = "1H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity",
abstract = "We used quantitative nuclear magnetic resonance analyses to measure the contents of major constituents of Acorus rhizome materials used as herbal drugs. The inhibitory effects of crude n-hexane extracts and their individual constituents on in vitro acetylcholine esterase activity were evaluated. The crude extracts had unexpectedly weak inhibitory effects (46-64{\%} inhibition at 1.0 mg/mL), despite the high content (46-64{\%}) of β-asarone, which independently had a potent effect (IC50 2.9 µM [0.61 µg/mL]). Further investigation revealed participation of eudesmin A, a lignan constituent, in the suppression of the inhibitory effect of β-asarone.",
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author = "Lynn, {Nwe Haymar} and Linn, {Thein Zaw} and Cui Yanmei and Yuuki Shimozu and Shoko Taniguchi and Tsutomu Hatano",
year = "2019",
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T1 - 1H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity

AU - Lynn, Nwe Haymar

AU - Linn, Thein Zaw

AU - Yanmei, Cui

AU - Shimozu, Yuuki

AU - Taniguchi, Shoko

AU - Hatano, Tsutomu

PY - 2019/5/1

Y1 - 2019/5/1

N2 - We used quantitative nuclear magnetic resonance analyses to measure the contents of major constituents of Acorus rhizome materials used as herbal drugs. The inhibitory effects of crude n-hexane extracts and their individual constituents on in vitro acetylcholine esterase activity were evaluated. The crude extracts had unexpectedly weak inhibitory effects (46-64% inhibition at 1.0 mg/mL), despite the high content (46-64%) of β-asarone, which independently had a potent effect (IC50 2.9 µM [0.61 µg/mL]). Further investigation revealed participation of eudesmin A, a lignan constituent, in the suppression of the inhibitory effect of β-asarone.

AB - We used quantitative nuclear magnetic resonance analyses to measure the contents of major constituents of Acorus rhizome materials used as herbal drugs. The inhibitory effects of crude n-hexane extracts and their individual constituents on in vitro acetylcholine esterase activity were evaluated. The crude extracts had unexpectedly weak inhibitory effects (46-64% inhibition at 1.0 mg/mL), despite the high content (46-64%) of β-asarone, which independently had a potent effect (IC50 2.9 µM [0.61 µg/mL]). Further investigation revealed participation of eudesmin A, a lignan constituent, in the suppression of the inhibitory effect of β-asarone.

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