16S rRNA gene sequence of Rubrobacter radiotolerans and its phylogenetic alignment with members of the genus Arthrobacter, gram-positive bacteria, and members of the family Deinococcaceae

Javed Kausar, Yoshihiko Ohyama, Hiroaki Terato, Hiroshi Ide, Osamu Yamamoto

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11 Citations (Scopus)

Abstract

The nearly complete sequence of the 16S rRNA gene of an extremely highly radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotolerans based on chemical characteristics), was determined by PCR amplification of the genomic DNA followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. The sequence was aligned with the sequences of members of the genus Arthrobacter and also with the sequences of representatives of the gram-positive bacteria having high G+C contents and the family Deinoroccaceae (radioresistant micrococci and their relatives). The results of our phylogenetic analysis confirmed that R. radiotolerans is not a member of the Arthrobaeter group and thus supported the previous reclassification. Moreover, although it is radioresistant and has a high G+C content, R. radiotolerans is more closely related to the gram- positive bacteria with high G+C contents than to the radioresistant members of the Deinococcaceae.

Original languageEnglish
Pages (from-to)684-686
Number of pages3
JournalInternational Journal of Systematic Bacteriology
Volume47
Issue number3
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

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Rubrobacter radiotolerans
Arthrobacter
Base Composition
Gram-Positive Bacteria
Gram-positive bacteria
rRNA Genes
ribosomal RNA
phylogenetics
nucleotide sequences
bacterium
gene
phylogeny
Dideoxynucleotides
Micrococcus
Micrococcaceae
Organism Cloning
amplification
genomics
taxonomic revisions
Bacteria

ASJC Scopus subject areas

  • Microbiology
  • Immunology

Cite this

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title = "16S rRNA gene sequence of Rubrobacter radiotolerans and its phylogenetic alignment with members of the genus Arthrobacter, gram-positive bacteria, and members of the family Deinococcaceae",
abstract = "The nearly complete sequence of the 16S rRNA gene of an extremely highly radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotolerans based on chemical characteristics), was determined by PCR amplification of the genomic DNA followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. The sequence was aligned with the sequences of members of the genus Arthrobacter and also with the sequences of representatives of the gram-positive bacteria having high G+C contents and the family Deinoroccaceae (radioresistant micrococci and their relatives). The results of our phylogenetic analysis confirmed that R. radiotolerans is not a member of the Arthrobaeter group and thus supported the previous reclassification. Moreover, although it is radioresistant and has a high G+C content, R. radiotolerans is more closely related to the gram- positive bacteria with high G+C contents than to the radioresistant members of the Deinococcaceae.",
author = "Javed Kausar and Yoshihiko Ohyama and Hiroaki Terato and Hiroshi Ide and Osamu Yamamoto",
year = "1997",
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doi = "10.1099/00207713-47-3-684",
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volume = "47",
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T1 - 16S rRNA gene sequence of Rubrobacter radiotolerans and its phylogenetic alignment with members of the genus Arthrobacter, gram-positive bacteria, and members of the family Deinococcaceae

AU - Kausar, Javed

AU - Ohyama, Yoshihiko

AU - Terato, Hiroaki

AU - Ide, Hiroshi

AU - Yamamoto, Osamu

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The nearly complete sequence of the 16S rRNA gene of an extremely highly radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotolerans based on chemical characteristics), was determined by PCR amplification of the genomic DNA followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. The sequence was aligned with the sequences of members of the genus Arthrobacter and also with the sequences of representatives of the gram-positive bacteria having high G+C contents and the family Deinoroccaceae (radioresistant micrococci and their relatives). The results of our phylogenetic analysis confirmed that R. radiotolerans is not a member of the Arthrobaeter group and thus supported the previous reclassification. Moreover, although it is radioresistant and has a high G+C content, R. radiotolerans is more closely related to the gram- positive bacteria with high G+C contents than to the radioresistant members of the Deinococcaceae.

AB - The nearly complete sequence of the 16S rRNA gene of an extremely highly radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotolerans based on chemical characteristics), was determined by PCR amplification of the genomic DNA followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. The sequence was aligned with the sequences of members of the genus Arthrobacter and also with the sequences of representatives of the gram-positive bacteria having high G+C contents and the family Deinoroccaceae (radioresistant micrococci and their relatives). The results of our phylogenetic analysis confirmed that R. radiotolerans is not a member of the Arthrobaeter group and thus supported the previous reclassification. Moreover, although it is radioresistant and has a high G+C content, R. radiotolerans is more closely related to the gram- positive bacteria with high G+C contents than to the radioresistant members of the Deinococcaceae.

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