16S rRNA gene sequence of Rubrobacter radiotolerans and its phylogenetic alignment with members of the genus Arthrobacter, gram-positive bacteria, and members of the family Deinococcaceae

Javed Kausar, Yoshihiko Ohyama, Hiroaki Terato, Hiroshi Ide, Osamu Yamamoto

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The nearly complete sequence of the 16S rRNA gene of an extremely highly radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotolerans based on chemical characteristics), was determined by PCR amplification of the genomic DNA followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. The sequence was aligned with the sequences of members of the genus Arthrobacter and also with the sequences of representatives of the gram-positive bacteria having high G+C contents and the family Deinoroccaceae (radioresistant micrococci and their relatives). The results of our phylogenetic analysis confirmed that R. radiotolerans is not a member of the Arthrobaeter group and thus supported the previous reclassification. Moreover, although it is radioresistant and has a high G+C content, R. radiotolerans is more closely related to the gram- positive bacteria with high G+C contents than to the radioresistant members of the Deinococcaceae.

Original languageEnglish
Pages (from-to)684-686
Number of pages3
JournalInternational Journal of Systematic Bacteriology
Issue number3
Publication statusPublished - Jul 1997
Externally publishedYes


ASJC Scopus subject areas

  • Microbiology
  • Immunology

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