Abstract
Ca2+/calmodulin-dependent protein kinase kinase α (CaMKKα) plays critical roles in the modulation of neuronal cell survival as well as many other cellular activities. Here we show that 14-3-3 proteins directly regulate CaMKKα when the enzyme is phosphorylated by protein kinase A on either Ser74 or Ser475. Mutational analysis revealed that these two serines are both functional: the CaMKKα mutant with a mutation at either of these residues, but not the double mutant, was inhibited significantly by 14-3-3. The mode of regulation described herein differs the recently described mode of 14-3-3 regulation of CaMKKα.
Original language | English |
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Pages (from-to) | 661-665 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 582 |
Issue number | 5 |
DOIs | |
Publication status | Published - Mar 5 2008 |
Externally published | Yes |
Keywords
- 14-3-3 Protein
- Calmodulin
- Protein kinase
- Proteomics
- Signal transduction
- cAMP
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology