1-Fluoro-2,4-dinitrobenzene and its derivatives act as secretagogues on rodent mast cells

Yohei Manabe, Marie Yoshimura, Kazuma Sakamaki, Asuka Inoue, Aya Kakinoki, Satoshi Hokari, Mariko Sakanaka, Junken Aoki, Hiroyuki Miyachi, Kazuyuki Furuta, Satoshi Tanaka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Accumulating evidence suggests that activated mast cells are involved in contact hypersensitivity, although the precise mechanisms of their activation are still not completely understood. We investigated the potential of common experimental allergens to induce mast cell activation using murine bone marrow-derived cultured mast cells and rat peritoneal mast cells. Among these allergens, 1-chloro-2,4-dinitrobenzene and 1-fluoro-2,4-dinirobenzene (DNFB) were found to induce degranulation of rat peritoneal mast cells. DNFB-induced degranulation is accompanied by cytosolic Ca2+ mobilization and is significantly inhibited by pertussis toxin, U73122 (a phospholipase C inhibitor), and BAPTA (a Ca2+ chelator), raising the possibility that DNFB acts on the G protein-coupled receptors and activates Gi, which induces activation of phospholipase C, as well as known mast cell secretagogues, such as compound 48/80. DNFB could induce mast cell degranulation in the absence of serum proteins and IgE. Structure-activity relationship analyses revealed an inverse correlation between the degree of degranulation and the electron density of the C1 carbon of the DNFB derivatives. These findings raise a possibility that DNFB functions as a potent contact allergen through induction of cutaneous mast cell degranulation.

Original languageEnglish
Pages (from-to)60-67
Number of pages8
JournalEuropean Journal of Immunology
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Dinitrofluorobenzene
Mast Cells
Rodentia
Allergens
Cell Degranulation
Type C Phospholipases
p-Methoxy-N-methylphenethylamine
Dinitrochlorobenzene
Contact Dermatitis
Pertussis Toxin
Structure-Activity Relationship
Chelating Agents
G-Protein-Coupled Receptors
Immunoglobulin E
Blood Proteins
Cultured Cells
Carbon
Bone Marrow
Electrons
Skin

Keywords

  • 1-Fluoro-2,4-dinitrobenzene
  • Contact hypersensitivity
  • Histamine
  • Inflammation
  • Mast cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Manabe, Y., Yoshimura, M., Sakamaki, K., Inoue, A., Kakinoki, A., Hokari, S., ... Tanaka, S. (2017). 1-Fluoro-2,4-dinitrobenzene and its derivatives act as secretagogues on rodent mast cells. European Journal of Immunology, 47(1), 60-67. https://doi.org/10.1002/eji.201646536

1-Fluoro-2,4-dinitrobenzene and its derivatives act as secretagogues on rodent mast cells. / Manabe, Yohei; Yoshimura, Marie; Sakamaki, Kazuma; Inoue, Asuka; Kakinoki, Aya; Hokari, Satoshi; Sakanaka, Mariko; Aoki, Junken; Miyachi, Hiroyuki; Furuta, Kazuyuki; Tanaka, Satoshi.

In: European Journal of Immunology, Vol. 47, No. 1, 01.01.2017, p. 60-67.

Research output: Contribution to journalArticle

Manabe, Y, Yoshimura, M, Sakamaki, K, Inoue, A, Kakinoki, A, Hokari, S, Sakanaka, M, Aoki, J, Miyachi, H, Furuta, K & Tanaka, S 2017, '1-Fluoro-2,4-dinitrobenzene and its derivatives act as secretagogues on rodent mast cells', European Journal of Immunology, vol. 47, no. 1, pp. 60-67. https://doi.org/10.1002/eji.201646536
Manabe, Yohei ; Yoshimura, Marie ; Sakamaki, Kazuma ; Inoue, Asuka ; Kakinoki, Aya ; Hokari, Satoshi ; Sakanaka, Mariko ; Aoki, Junken ; Miyachi, Hiroyuki ; Furuta, Kazuyuki ; Tanaka, Satoshi. / 1-Fluoro-2,4-dinitrobenzene and its derivatives act as secretagogues on rodent mast cells. In: European Journal of Immunology. 2017 ; Vol. 47, No. 1. pp. 60-67.
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