1'-acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin

Akira Murakami, Shin Ohura, Yoshimasa Nakamura, Koichi Koshimizu, Hajime Ohigashi

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The anti-tumor-promoting activity of 1'-acetoxychavicol acetate (ACA) was examined in a two-stage carcinogenesis experiment in ICR mouse skin using 7,12-dimethylbenz[a]anthracene (0.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA; 1.6 nmol). Topical application of ACA (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice: inhibition ratios 90% (p <0.001) and 42% (p <0.005), respectively. ACA even at a dose equimolar to TPA (1.6 nmol) significantly reduced the average number of tumors per mouse: inhibitory ratio 44% (p <0.05). ACA potently inhibited TPA-induced superoxide (O2-) generation in differentiated HL-60 cells (IC50 = 4.3 μM) and suppressed the lipid hydroperoxide formation by 42% (p <0.001) in the ethyl linoleate autoxidation test.

Original languageEnglish
Pages (from-to)386-391
Number of pages6
JournalOncology
Volume53
Issue number5
Publication statusPublished - Sep 1996
Externally publishedYes

Fingerprint

Inbred ICR Mouse
Tetradecanoylphorbol Acetate
Superoxides
Skin
Acetates
Neoplasms
Lipid Peroxides
HL-60 Cells
Inhibitory Concentration 50
Carcinogenesis
1'-acetoxychavicol acetate

Keywords

  • 1'-acetoxychavicol acetate
  • Antioxidant
  • Antitumor promoter
  • Cancer chemoprevention
  • HL-60 cells
  • Languas galanga
  • Mouse skin
  • Superoxide anion
  • Xanthine oxidase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

1'-acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin. / Murakami, Akira; Ohura, Shin; Nakamura, Yoshimasa; Koshimizu, Koichi; Ohigashi, Hajime.

In: Oncology, Vol. 53, No. 5, 09.1996, p. 386-391.

Research output: Contribution to journalArticle

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