β2-Glycoprotein I reactivity of monoclonal anticardiolipin antibodies from patients with the antiphospholipid syndrome

Kenji Ichikawa, Munther A. Khamashta, Takao Koike, Eiji Matsuura, Graham R V Hughes

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Objective. To elucidate the specificity of anticardiolipin antibodies (aCL) from patients with the antiphospholipid syndrome (APS) to various phospholipids (PLs), DNA, and β2-glycoprotein I (β2-GPI). Methods. Five monoclonal aCL were established from peripheral blood lymphocytes of 3 patients with the APS. The reactivity of monoclonal aCL with various PLs, with DNA, and with β2-GPI was examined by enzyme-linked immunosorbent assay (ELISA). Results. All of the monoclonal aCL bound to anionic PLs, only in the presence of β2-GPI. Neither monoclonal aCL nor β2-GPI bound to DNA. Monoclonal aCL bound to solid-phase β2-GPI on polystyrene ELISA plates that had carboxyl groups on their surface, but did not react with solid-phase β2-GPI on ordinary polystyrene plates. A mixture of β2-GPI and CL inhibited the binding of monoclonal aCL to β2-GPI, but CL or β2-GPI alone did not. Conclusion. Monoclonal aCL may recognize a cryptic epitope, which appears as a result of β2-GPI binding to anionic PLs or to polystyrene with carboxyl groups.

Original languageEnglish
Pages (from-to)1453-1461
Number of pages9
JournalArthritis and Rheumatism
Volume37
Issue number10
DOIs
Publication statusPublished - Oct 1994
Externally publishedYes

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Anticardiolipin Antibodies
Antiphospholipid Syndrome
Glycoproteins
Monoclonal Antibodies
Polystyrenes
Phospholipids
DNA
Enzyme-Linked Immunosorbent Assay
Epitopes
Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

β2-Glycoprotein I reactivity of monoclonal anticardiolipin antibodies from patients with the antiphospholipid syndrome. / Ichikawa, Kenji; Khamashta, Munther A.; Koike, Takao; Matsuura, Eiji; Hughes, Graham R V.

In: Arthritis and Rheumatism, Vol. 37, No. 10, 10.1994, p. 1453-1461.

Research output: Contribution to journalArticle

Ichikawa, Kenji ; Khamashta, Munther A. ; Koike, Takao ; Matsuura, Eiji ; Hughes, Graham R V. / β2-Glycoprotein I reactivity of monoclonal anticardiolipin antibodies from patients with the antiphospholipid syndrome. In: Arthritis and Rheumatism. 1994 ; Vol. 37, No. 10. pp. 1453-1461.
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