β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes

Kenji Mizuno, Hideo Kohka Takahashi, Hiromi Iwagaki, Goutaro Katsuno, Huda A.S.M. Kamurul, Satoru Ohtani, Shuji Mori, Tadashi Yoshino, Masahiro Nishibori, Noriaki Tanaka

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We examined the effects of β2-adrenergic receptor (β2-AR) agonists on monocyte-derived cytokines, interleukin (IL)-18 and IL-12 production in lipopolysaccharide (LPS)-stimulated monocytes derived from human peripheral blood mononuclear cells (PBMCs), as in vitro model of sepsis. The study found that β2-AR agonists inhibited IL-18 and IL-12 production in monocytes, and that AR agonist activity was antagonized by the selective β2-AR antagonist, butoxamine. The selective β2-AR agonists salbutamol and terbutaline induced a similar inhibitory pattern of IL-18 and IL-12 production. IL-12 production induced by LPS was inhibited by anti-IL-18 Ab, but IL-18 production by LPS was not inhibited by anti-IL-12 Ab, showing that LPS induced IL-18 production without IL-12 production. Therefore, the stimulation of β2-AR might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalImmunology Letters
Volume101
Issue number2
DOIs
Publication statusPublished - Nov 15 2005

Keywords

  • Adrenergic receptor
  • IL-12
  • IL-18

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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