β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes

Kenji Mizuno, Hideo Kohka Takahashi, Hiromi Iwagaki, Goutaro Katsuno, Huda A S M Kamurul, Satoru Ohtani, Shuji Mori, Tadashi Yoshino, Masahiro Nishibori, Noriaki Tanaka

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We examined the effects of β2-adrenergic receptor (β2-AR) agonists on monocyte-derived cytokines, interleukin (IL)-18 and IL-12 production in lipopolysaccharide (LPS)-stimulated monocytes derived from human peripheral blood mononuclear cells (PBMCs), as in vitro model of sepsis. The study found that β2-AR agonists inhibited IL-18 and IL-12 production in monocytes, and that AR agonist activity was antagonized by the selective β2-AR antagonist, butoxamine. The selective β2-AR agonists salbutamol and terbutaline induced a similar inhibitory pattern of IL-18 and IL-12 production. IL-12 production induced by LPS was inhibited by anti-IL-18 Ab, but IL-18 production by LPS was not inhibited by anti-IL-12 Ab, showing that LPS induced IL-18 production without IL-12 production. Therefore, the stimulation of β2-AR might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalImmunology Letters
Volume101
Issue number2
DOIs
Publication statusPublished - Nov 15 2005

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Interleukin-18
Interleukin-12
Adrenergic Receptors
Lipopolysaccharides
Monocytes
Adrenergic Agonists
Sepsis
Butoxamine
Terbutaline
Adrenergic Antagonists
Albuterol
Blood Cells
Cytokines

Keywords

  • Adrenergic receptor
  • IL-12
  • IL-18

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Mizuno, K., Takahashi, H. K., Iwagaki, H., Katsuno, G., Kamurul, H. A. S. M., Ohtani, S., ... Tanaka, N. (2005). β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes. Immunology Letters, 101(2), 168-172. https://doi.org/10.1016/j.imlet.2005.05.008

β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes. / Mizuno, Kenji; Takahashi, Hideo Kohka; Iwagaki, Hiromi; Katsuno, Goutaro; Kamurul, Huda A S M; Ohtani, Satoru; Mori, Shuji; Yoshino, Tadashi; Nishibori, Masahiro; Tanaka, Noriaki.

In: Immunology Letters, Vol. 101, No. 2, 15.11.2005, p. 168-172.

Research output: Contribution to journalArticle

Mizuno, K, Takahashi, HK, Iwagaki, H, Katsuno, G, Kamurul, HASM, Ohtani, S, Mori, S, Yoshino, T, Nishibori, M & Tanaka, N 2005, 'β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes', Immunology Letters, vol. 101, no. 2, pp. 168-172. https://doi.org/10.1016/j.imlet.2005.05.008
Mizuno K, Takahashi HK, Iwagaki H, Katsuno G, Kamurul HASM, Ohtani S et al. β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes. Immunology Letters. 2005 Nov 15;101(2):168-172. https://doi.org/10.1016/j.imlet.2005.05.008
Mizuno, Kenji ; Takahashi, Hideo Kohka ; Iwagaki, Hiromi ; Katsuno, Goutaro ; Kamurul, Huda A S M ; Ohtani, Satoru ; Mori, Shuji ; Yoshino, Tadashi ; Nishibori, Masahiro ; Tanaka, Noriaki. / β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes. In: Immunology Letters. 2005 ; Vol. 101, No. 2. pp. 168-172.
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