Abstract
Deletion of integrin-β1 (Itgb1) in the kidney collecting system led to progressive renal dysfunction and polyuria. The defect in the concentrating ability of the kidney was concomitant with decreased medullary collecting duct expression of aquaporin-2 and arginine vasopressin receptor 2, while histological examination revealed hypoplastic renal medullary collecting ducts characterized by increased apoptosis, ectasia and cyst formation. In addition, a range of defects from small kidneys with cysts and dilated tubules to bilateral renal agenesis was observed. This was likely due to altered growth and branching morphogenesis of the ureteric bud (the progenitor tissue of the renal collecting system), despite the apparent ability of the ureteric budderived cells to induce differentiation of the metanephric mesenchyme. These data not only support a role for Itgb1 in the development of the renal collecting system but also raise the possibility that Itgb1 links morphogenesis to terminal differentiation and ultimately collecting duct function and/or maintenance.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Renal Physiology |
| Volume | 297 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2009 |
| Externally published | Yes |
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Keywords
- Branching morphogenesis
- Cystic kidney disease
- Diabetes insipidus
- Metanephric mesenchyme
- Polyuria
- Renal failure
- Ureteric bud
ASJC Scopus subject areas
- Physiology
- Urology
- Medicine(all)
Cite this
β1-Integrin is required for kidney collecting duct morphogenesis and maintenance of renal function. / Wu, Wei; Kitamura, Shinji; Truong, David M.; Rieg, Timo; Vallon, Volker; Sakurai, Hiroyuki; Bush, Kevin T.; Vera, David R.; Ross, Robert S.; Nigam, Sanjay K.
In: American Journal of Physiology - Renal Physiology, Vol. 297, No. 1, 07.2009.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - β1-Integrin is required for kidney collecting duct morphogenesis and maintenance of renal function
AU - Wu, Wei
AU - Kitamura, Shinji
AU - Truong, David M.
AU - Rieg, Timo
AU - Vallon, Volker
AU - Sakurai, Hiroyuki
AU - Bush, Kevin T.
AU - Vera, David R.
AU - Ross, Robert S.
AU - Nigam, Sanjay K.
PY - 2009/7
Y1 - 2009/7
N2 - Deletion of integrin-β1 (Itgb1) in the kidney collecting system led to progressive renal dysfunction and polyuria. The defect in the concentrating ability of the kidney was concomitant with decreased medullary collecting duct expression of aquaporin-2 and arginine vasopressin receptor 2, while histological examination revealed hypoplastic renal medullary collecting ducts characterized by increased apoptosis, ectasia and cyst formation. In addition, a range of defects from small kidneys with cysts and dilated tubules to bilateral renal agenesis was observed. This was likely due to altered growth and branching morphogenesis of the ureteric bud (the progenitor tissue of the renal collecting system), despite the apparent ability of the ureteric budderived cells to induce differentiation of the metanephric mesenchyme. These data not only support a role for Itgb1 in the development of the renal collecting system but also raise the possibility that Itgb1 links morphogenesis to terminal differentiation and ultimately collecting duct function and/or maintenance.
AB - Deletion of integrin-β1 (Itgb1) in the kidney collecting system led to progressive renal dysfunction and polyuria. The defect in the concentrating ability of the kidney was concomitant with decreased medullary collecting duct expression of aquaporin-2 and arginine vasopressin receptor 2, while histological examination revealed hypoplastic renal medullary collecting ducts characterized by increased apoptosis, ectasia and cyst formation. In addition, a range of defects from small kidneys with cysts and dilated tubules to bilateral renal agenesis was observed. This was likely due to altered growth and branching morphogenesis of the ureteric bud (the progenitor tissue of the renal collecting system), despite the apparent ability of the ureteric budderived cells to induce differentiation of the metanephric mesenchyme. These data not only support a role for Itgb1 in the development of the renal collecting system but also raise the possibility that Itgb1 links morphogenesis to terminal differentiation and ultimately collecting duct function and/or maintenance.
KW - Branching morphogenesis
KW - Cystic kidney disease
KW - Diabetes insipidus
KW - Metanephric mesenchyme
KW - Polyuria
KW - Renal failure
KW - Ureteric bud
UR - http://www.scopus.com/inward/record.url?scp=67650650694&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650650694&partnerID=8YFLogxK
U2 - 10.1152/ajprenal.90260.2008
DO - 10.1152/ajprenal.90260.2008
M3 - Article
C2 - 19439520
AN - SCOPUS:67650650694
VL - 297
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
SN - 0363-6127
IS - 1
ER -