β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification

Chika Ikenoya, Shota Takemi, Arisa Kaminoda, Sayaka Aizawa, Shiomi Ojima, Zhi Gong, Rakhi Chacrabati, Daisuke Kondo, Reiko Wada, Toru Tanaka, Sachiko Tsuda, Takafumi Sakai, Ichiro Sakata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ghrelin is a unique fatty acid-modified peptide hormone produced in the stomach and has important roles in energy homeostasis and gastrointestinal motility. However, the medium-chain fatty acid source for ghrelin acyl-modification is not known. We found that a fat-free diet and the removal of intestinal microbiota did not decrease acyl-ghrelin production in the stomach or plasma acyl-ghrelin levels in mice. RT-PCR analysis showed that genes involving fatty acid synthesis, metabolism, and transport were expressed in pancreas-derived ghrelinoma (PG-1) cells. Treatment with an irreversible inhibitor of carnitine palmitoyltransferase-1 (CPT-1) strongly decreased acylated ghrelin levels but did not affect ghrelin or ghrelin o-acyl transferase (GOAT) mRNA levels in PG-1 cells. Our results suggest that the medium-chain fatty acid used for the acyl-modification of ghrelin is produced in ghrelin-producing cells themselves by β-oxidation of long-chain fatty acids provided from the circulation.

Original languageEnglish
Article number9176
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Ghrelin
Fatty Acids
Stomach
Carnitine O-Palmitoyltransferase
Fat-Restricted Diet
Gastrointestinal Motility
Peptide Hormones
Transferases
Pancreas
Homeostasis
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • General

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β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification. / Ikenoya, Chika; Takemi, Shota; Kaminoda, Arisa; Aizawa, Sayaka; Ojima, Shiomi; Gong, Zhi; Chacrabati, Rakhi; Kondo, Daisuke; Wada, Reiko; Tanaka, Toru; Tsuda, Sachiko; Sakai, Takafumi; Sakata, Ichiro.

In: Scientific Reports, Vol. 8, No. 1, 9176, 01.12.2018.

Research output: Contribution to journalArticle

Ikenoya, C, Takemi, S, Kaminoda, A, Aizawa, S, Ojima, S, Gong, Z, Chacrabati, R, Kondo, D, Wada, R, Tanaka, T, Tsuda, S, Sakai, T & Sakata, I 2018, 'β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification', Scientific Reports, vol. 8, no. 1, 9176. https://doi.org/10.1038/s41598-018-27458-2
Ikenoya, Chika ; Takemi, Shota ; Kaminoda, Arisa ; Aizawa, Sayaka ; Ojima, Shiomi ; Gong, Zhi ; Chacrabati, Rakhi ; Kondo, Daisuke ; Wada, Reiko ; Tanaka, Toru ; Tsuda, Sachiko ; Sakai, Takafumi ; Sakata, Ichiro. / β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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