β-Carotene Probes the Energy Transfer Pathway in the Photosystem II Core Complex

Yusuke Yoneda, Yutaka Nagasawa, Yasufumi Umena, Hiroshi Miyasaka

Research output: Contribution to journalArticle

Abstract

The dynamics of the intact photosystem II core complex (PSII-CC) has been investigated extensively to elucidate its excellent photofunction. However, it is significantly difficult to observe the primary photosynthetic processes in PSII-CC because a vast number of chlorophylls (Chl) in the core complex show similar spectral features. In the present work, the dynamics of the energy transfer (ET) from β-carotene (Bcr) in intact PSII-CC followed by charge separation (CS) at the reaction center (RC) with different excitation wavelengths were compared. Upon excitation at 510 nm, which selectively excites Bcr (Bcr651) inside of the D1-D2 RC, the pheophytin anion absorption band appeared within 9.6 ps. On the other hand, upon excitation at 490 nm, mainly exciting unspecified Bcr in the antenna complex, the anion band appeared after 20 ps. These excitation wavelength dependence experiments revealed a new ET pathway of PSII-CC, which indicates that the initial CS of PSII-CC is limited by ET to the RC.

Original languageEnglish
Pages (from-to)3710-3714
Number of pages5
JournalJournal of Physical Chemistry Letters
DOIs
Publication statusPublished - Jan 1 2019

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carotene
Photosystem II Protein Complex
Carotenoids
Energy transfer
energy transfer
probes
Negative ions
Wavelength
Anions
Chlorophyll
polarization (charge separation)
Pheophytins
excitation
Absorption spectra
Antennas
anions
chlorophylls
wavelengths
antennas
Experiments

ASJC Scopus subject areas

  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

β-Carotene Probes the Energy Transfer Pathway in the Photosystem II Core Complex. / Yoneda, Yusuke; Nagasawa, Yutaka; Umena, Yasufumi; Miyasaka, Hiroshi.

In: Journal of Physical Chemistry Letters, 01.01.2019, p. 3710-3714.

Research output: Contribution to journalArticle

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