Abstract
Dietary intake of quercetin is suggested to be potentially beneficial for the prevention of various diseases. We examined the effect of α-oligoglucosylation of the sugar moiety of quercetin monoglucoside on its bioavailability in humans. Enzymatically modified isoquercitrin (EMIQ) was prepared by enzymatic deglycosylation and the subsequent of α-oligoglucosylation of quercetin 3-O-β-rutinode (rutin). The plasma level of quercetin metabolites was instantly increased by oral intake of EMIQ and its absorption efficiency was significantly higher than that of isoquercitrin (quercetin 3-O-β-glucoside; Q3G), and rutin. The profile of plasma quercetin metabolites after EMIQ consumption did not differ from that after Q3G consumption. The apparent log P of EMIQ indicated that EMIQ is more hydrophilic than Q3G but less than quercetin 3,4'-O-β-diglucoside. These data indicated that enzymatic α-oligoglucosylation to the sugar moiety is effective for enhancing the bioavailability of quercetin glucosides in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 91-97 |
| Number of pages | 7 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 501 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Sep 2010 |
| Externally published | Yes |
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Keywords
- α-Glucosylation
- Bioavailability
- EMIQ
- Human
- Quercetin
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
- Medicine(all)
Cite this
α-Oligoglucosylation of a sugar moiety enhances the bioavailability of quercetin glucosides in humans. / Murota, Kaeko; Matsuda, Namiko; Kashino, Yasuaki; Fujikura, Yutaka; Nakamura, Toshiyuki; Kato, Yoji; Shimizu, Ryosuke; Okuyama, Syuji; Tanaka, Hisashi; Koda, Takatoshi; Sekido, Keiko; Terao, Junji.
In: Archives of Biochemistry and Biophysics, Vol. 501, No. 1, 09.2010, p. 91-97.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - α-Oligoglucosylation of a sugar moiety enhances the bioavailability of quercetin glucosides in humans
AU - Murota, Kaeko
AU - Matsuda, Namiko
AU - Kashino, Yasuaki
AU - Fujikura, Yutaka
AU - Nakamura, Toshiyuki
AU - Kato, Yoji
AU - Shimizu, Ryosuke
AU - Okuyama, Syuji
AU - Tanaka, Hisashi
AU - Koda, Takatoshi
AU - Sekido, Keiko
AU - Terao, Junji
PY - 2010/9
Y1 - 2010/9
N2 - Dietary intake of quercetin is suggested to be potentially beneficial for the prevention of various diseases. We examined the effect of α-oligoglucosylation of the sugar moiety of quercetin monoglucoside on its bioavailability in humans. Enzymatically modified isoquercitrin (EMIQ) was prepared by enzymatic deglycosylation and the subsequent of α-oligoglucosylation of quercetin 3-O-β-rutinode (rutin). The plasma level of quercetin metabolites was instantly increased by oral intake of EMIQ and its absorption efficiency was significantly higher than that of isoquercitrin (quercetin 3-O-β-glucoside; Q3G), and rutin. The profile of plasma quercetin metabolites after EMIQ consumption did not differ from that after Q3G consumption. The apparent log P of EMIQ indicated that EMIQ is more hydrophilic than Q3G but less than quercetin 3,4'-O-β-diglucoside. These data indicated that enzymatic α-oligoglucosylation to the sugar moiety is effective for enhancing the bioavailability of quercetin glucosides in humans.
AB - Dietary intake of quercetin is suggested to be potentially beneficial for the prevention of various diseases. We examined the effect of α-oligoglucosylation of the sugar moiety of quercetin monoglucoside on its bioavailability in humans. Enzymatically modified isoquercitrin (EMIQ) was prepared by enzymatic deglycosylation and the subsequent of α-oligoglucosylation of quercetin 3-O-β-rutinode (rutin). The plasma level of quercetin metabolites was instantly increased by oral intake of EMIQ and its absorption efficiency was significantly higher than that of isoquercitrin (quercetin 3-O-β-glucoside; Q3G), and rutin. The profile of plasma quercetin metabolites after EMIQ consumption did not differ from that after Q3G consumption. The apparent log P of EMIQ indicated that EMIQ is more hydrophilic than Q3G but less than quercetin 3,4'-O-β-diglucoside. These data indicated that enzymatic α-oligoglucosylation to the sugar moiety is effective for enhancing the bioavailability of quercetin glucosides in humans.
KW - α-Glucosylation
KW - Bioavailability
KW - EMIQ
KW - Human
KW - Quercetin
UR - http://www.scopus.com/inward/record.url?scp=77956106562&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956106562&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2010.06.036
DO - 10.1016/j.abb.2010.06.036
M3 - Article
VL - 501
SP - 91
EP - 97
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 1
ER -