α-melanocyte-stimulating hormone stimulates prolactin secretion through melanocortin-3 receptors expressed in mammotropes in the mouse pituitary

Ryusei Matsumura, Chika Takagi, Tomoshi Kakeya, Kiyoshi Okuda, Sakae Takeuchi, Sumio Takahashi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The intermediate lobe of rodent pituitaries is involved in the regulation of prolactin (PRL) secretion from the anterior lobe. In a previous study, we demonstrated the stimulatory effect of α-melanocyte-stimulating hormone (α-MSH) on PRL release and the expression of melanocortin-3 receptors (MC3-Rs) in cultured mouse pituitary cells. The aim of the present study was to clarify whether α-MSH directly stimulates PRL release through the MC3-Rs by determining the cell type of MC3-R-expressing cells in the mouse pituitary anterior lobe. Northern blot analysis revealed a 2.7-kb transcript for MC3-R mRNA in the anterior and neurointermediate lobes of pituitary glands of adult male and female mice. Dual cellular localization of MC3-R mRNA and PRL or growth hormone (GH) in the mouse pituitary glands was performed by in situ hybridization analysis of MC3-R mRNA followed by immunocytochemical detection of PRL or GH. MC3-R mRNA was detected in most mammotropes and some somatotropes. α-MSH increased PRL release and stimulated DNA replication in mammotropes, and these effects were blocked by SHU9119, an antagonist of MC3-R and MC4-R. These results indicate that α-MSH stimulates PRL release and proliferation of mammotropes through MC3-Rs, and suggest that α-MSH from intermediate lobes can regulate mammotrope functions in the mouse pituitary.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalNeuroendocrinology
Volume78
Issue number2
DOIs
Publication statusPublished - 2003

Fingerprint

Receptor, Melanocortin, Type 3
Melanocyte-Stimulating Hormones
Prolactin
Messenger RNA
Anterior Pituitary Gland
Pituitary Gland
Growth Hormone
Intermediate Pituitary Gland
DNA Replication
Northern Blotting
In Situ Hybridization
Rodentia

Keywords

  • Immunocytochemisry
  • Lactotropes
  • Melanocortin receptors
  • Melanocyte-stimulating hormone
  • Mouse
  • Prolactin
  • Somatotropes

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

α-melanocyte-stimulating hormone stimulates prolactin secretion through melanocortin-3 receptors expressed in mammotropes in the mouse pituitary. / Matsumura, Ryusei; Takagi, Chika; Kakeya, Tomoshi; Okuda, Kiyoshi; Takeuchi, Sakae; Takahashi, Sumio.

In: Neuroendocrinology, Vol. 78, No. 2, 2003, p. 96-104.

Research output: Contribution to journalArticle

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