Tsutomu Ono &ndash; Research Output

2005

68 Citations (Scopus)

Development of mammalian cell-enclosing subsieve-size agarose capsules (

Sakai, S., Kawabata, K., Ono, T., Ijima, H. & Kawakami, K., Aug 2005, In : Biomaterials. 26, 23, p. 4786-4792 7 p.

Research output: Contribution to journal › Article

Sepharose

Paraffins

Capsules

Mineral Oil

Cells

3 Citations (Scopus)

Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity

Takei, T., Ijima, H., Sakai, S., Ono, T. & Kawakami, K., Nov 20 2005, In : Journal of Chemical Engineering of Japan. 38, 11, p. 913-917 5 p.

Research output: Contribution to journal › Article

Fibroblast Growth Factor 2

Fibroblasts

Glycogen

Scaffolds

Rats

10 Citations (Scopus)

Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces

Ijima, H., Murakami, S., Matsuo, T., Takei, T., Ono, T. & Kawakami, K., Aug 2005, In : Journal of Artificial Organs. 8, 2, p. 104-109 6 p.

Research output: Contribution to journal › Article

Tissue culture

Polystyrenes

Bone Marrow Cells

Liver

Rats

11 Citations (Scopus)

Higher viscous solution induces smaller droplets for cell-enclosing capsules in a co-flowing stream

Sakai, S., Kawabata, K., Ono, T., Ijima, H. & Kawakami, K., May 2005, In : Biotechnology Progress. 21, 3, p. 994-997 4 p.

Research output: Contribution to journal › Article

droplets

Capsules

Viscosity

viscosity

aqueous solutions

10 Citations (Scopus)

Nonaggregating refolding of ribonuclease A using reverse micellar dialysis

Ono, T., Nagatomo, M., Nagao, T., Ijima, H. & Kawakami, K., Feb 5 2005, In : Biotechnology and Bioengineering. 89, 3, p. 290-295 6 p.

Research output: Contribution to journal › Article

Pancreatic Ribonuclease

Dialysis

Glutathione Disulfide

Protein Refolding

Ultrafiltration

18 Citations (Scopus)

Transition of mechanical property of porous alginate scaffold with cells during culture period

Sakai, S., Masuhara, H., Yamada, Y., Ono, T., Ijima, H. & Kawakami, K., 2005, In : Journal of Bioscience and Bioengineering. 100, 1, p. 127-129 3 p.

Research output: Contribution to journal › Article

Alginate

Scaffolds (biology)

Cell culture

Cell Culture Techniques

Scaffolds

2004

14 Citations (Scopus)

Behavior of enclosed sol- and gel-alginates in vivo

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., Dec 2004, In : Biochemical Engineering Journal. 22, 1, p. 19-24 6 p.

Research output: Contribution to journal › Article

Alginates

Alginate

Polymethyl Methacrylate

Sols

Gels

14 Citations (Scopus)

Development of co-culture system of hepatocytes with bone marrow cells for expression and maintenance of hepatic functions

Murakami, S., Ijima, H., Ono, T. & Kawakami, K., Feb 2004, In : International Journal of Artificial Organs. 27, 2, p. 118-126 9 p.

Research output: Contribution to journal › Article

Coculture Techniques

Cell culture

Bone Marrow Cells

Hepatocytes

Bone

19 Citations (Scopus)

DNA extraction by cationic reverse micelles

Goto, M., Momota, A. & Ono, T., May 2004, In : Journal of Chemical Engineering of Japan. 37, 5, p. 662-668 7 p.

Research output: Contribution to journal › Article

Micelles

DNA

Cationic surfactants

Surface-Active Agents

Surface active agents

Erratum: Hydrothermal synthesis of porous hydroxyapatite plates from gypsum: Use as an animal cell culture substratum [Journal of Chemical Engineering of Japan Vol. 37, No. 4, pp. 531-535]

Ijima, H., Nakashima, M., Furuta, S., Ono, T. & Kawakami, K., Sep 2004, In : Journal of Chemical Engineering of Japan. 37, 9, p. 1183 1 p.

Research output: Contribution to journal › Article

Animal cell culture

Hydrothermal synthesis

Gypsum

Chemical engineering

Hydroxyapatite

1 Citation (Scopus)

Hydrothermal synthesis of porous hydroxyapatite plates from gypsum: Use as an animal cell culture substratum

Ijima, H., Nakashima, M., Furuta, S., Ono, T. & Kawakami, K., Apr 2004, In : Journal of Chemical Engineering of Japan. 37, 4, p. 531-535 5 p.

Research output: Contribution to journal › Article

Animal cell culture

Calcium Sulfate

Hydrothermal synthesis

Gypsum

Durapatite

11 Citations (Scopus)

Hydroxyapatite for use as an animal cell culture substratum obtained by an alternate soaking process

Ijima, H., Ohchi, T., Ono, T. & Kawakami, K., Aug 15 2004, In : Biochemical Engineering Journal. 20, 2-3, p. 155-161 7 p.

Research output: Contribution to journal › Article

Animal cell culture

Durapatite

Hydroxyapatite

Cell Culture Techniques

CHO Cells

12 Citations (Scopus)

MIN6 cells-enclosing aminopropyl-silicate membrane templated by alginate gels differences in guluronic acid content

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., Feb 11 2004, In : International Journal of Pharmaceutics. 270, 1-2, p. 65-73 9 p.

Research output: Contribution to journal › Article

Silicates

Gels

Membranes

Capsules

Alginates

29 Citations (Scopus)

Preparation of Mammalian Cell-Enclosing Subsieve-Sized Capsules (<100 μm) in a Coflowing Stream

Sakai, S., Kawabata, K., Ono, T., Ijima, H. & Kawakami, K., Apr 20 2004, In : Biotechnology and Bioengineering. 86, 2, p. 168-173 6 p.

Research output: Contribution to journal › Article

Sodium alginate

Needles

Capsules

Cells

Mineral Oil

2003

7 Citations (Scopus)

Enhancement of protease activity in transesterification of glycidol with vinyl n-butyrate by entrapment into alkyl-substituted silicates and pretreatment with a substrate

Kawakami, K., Matsui, Y., Ono, T. & Ijima, H., Feb 2003, In : Biocatalysis and Biotransformation. 21, 1, p. 49-52 4 p.

Research output: Contribution to journal › Article

glycidol

Silicates

Butyrates

Transesterification

Peptide Hydrolases

11 Citations (Scopus)

Modification of porous aminopropyl-silicate microcapsule membrane by electrically-bonded external anionic polymers

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., 2003, In : Journal of Biomaterials Science, Polymer Edition. 14, 7, p. 643-652 10 p.

Research output: Contribution to journal › Article

Silicates

Capsules

Biocompatibility

Polymers

Membranes

27 Citations (Scopus)

Proliferation and insulin secretion function of mouse insulinoma cells encapsulated in alginate/sol-gel synthesized aminopropyl-silicate/alginate microcapsule

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., Sep 2003, In : Journal of Sol-Gel Science and Technology. 28, 2, p. 267-272 6 p.

Research output: Contribution to journal › Article

Silicates

insulin

secretions

Insulin

Alginate

2002

13 Citations (Scopus)

Activation of protease by sol-gel entrapment into organically modified hybrid silicates

Furukawa, S. Y., Ono, T., Ijima, H. & Kawakami, K., 2002, In : Biotechnology Letters. 24, 1, p. 13-16 4 p.

Research output: Contribution to journal › Article

Silicates

Polymethyl Methacrylate

Diatomaceous Earth

Sol-gels

Peptide Hydrolases

23 Citations (Scopus)

Alginate/aminopropyl-silicate/alginate membrane immunoisolatability and insulin secretion of encapsulated islets

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., 2002, In : Biotechnology Progress. 18, 2, p. 401-403 3 p.

Research output: Contribution to journal › Article

calcium alginate

Silicates

insulin secretion

alginates

silicates

19 Citations (Scopus)

Aminopropyl-silicate membrane for microcapsule-shaped bioartificial organs: Control of molecular permeability

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., Jun 15 2002, In : Journal of Membrane Science. 202, 1-2, p. 73-80 8 p.

Research output: Contribution to journal › Article

Bioartificial Organs

Silicates

organs

Capsules

Permeability

36 Citations (Scopus)

Effect of imprinting sol-gel immobilized lipase with chiral template substrates in esterification of (R)-(+)- and (S)-(-)-glycidol

Furukawa, S. Y., Ono, T., Ijima, H. & Kawakami, K., Mar 7 2002, In : Journal of Molecular Catalysis B: Enzymatic. 17, 1, p. 23-28 6 p.

Research output: Contribution to journal › Article

glycidol

Esterification

Lipases

Polymethyl Methacrylate

Lipase

76 Citations (Scopus)

In vitro and in vivo evaluation of alginate/sol-gel synthesized aminopropyl-silicate/alginate membrane for bioartificial pancreas

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., 2002, In : Biomaterials. 23, 21, p. 4177-4183 7 p.

Research output: Contribution to journal › Article

Silicates

Alginate

Polymethyl Methacrylate

Sol-gels

Pancreas

16 Citations (Scopus)

Permeability of alginate/sol-gel synthesized aminopropyl-silicate/alginate membrane templated by calcium-alginate gel

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., Aug 1 2002, In : Journal of Membrane Science. 205, 1-2, p. 183-189 7 p.

Research output: Contribution to journal › Article

Silicates

Alginate

Polymethyl Methacrylate

Sol-gels

calcium

1 Citation (Scopus)

Permeability of a sol-gel synthesized aminopropyl-silicate-titanate hybrid membrane for the microcapsule-shaped bioartificial pancreas

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., 2002, In : Journal of Artificial Organs. 5, 1, p. 54-59 6 p.

Research output: Contribution to journal › Article

Silicates

Alginate

Polymethyl Methacrylate

Capsules

Sol-gels

2001

18 Citations (Scopus)

Catalytic oxidation of o-phenylenediamine by cytochrome c encapsulated in reversed micelles

Ono, T., Kawakami, K., Goto, M. & Furusaki, S., Jan 22 2001, In : Journal of Molecular Catalysis B: Enzymatic. 11, 4-6, p. 955-959 5 p.

Research output: Contribution to journal › Article

Catalytic oxidation

Micelles

Cytochromes c

Proteins

Peroxidases

1 Citation (Scopus)

Control of Transport Characteristic of Membrane by Multi-layering of Polyelectrolyte Complex Toward Microcapsule-shaped Bioartificial Pancreas

Saka, S., Ono, T., Ijima, H. & Kawakami, K., 2001, In : Kagaku Kogaku Ronbunshu. 27, 2, p. 165-168 4 p.

Research output: Contribution to journal › Article

Polyelectrolytes

Capsules

Membranes

Alginate

Globulins

14 Citations (Scopus)

Enhancement of activity of sol-gel immobilized lipase in organic media by pretreatment with substrate analogues

Furukawa, S. Y., Ono, T., Ijima, H. & Kawakami, K., Sep 28 2001, In : Journal of Molecular Catalysis B: Enzymatic. 15, 1-3, p. 65-70 6 p.

Research output: Contribution to journal › Article

Lipases

Polymethyl Methacrylate

Lipase

Sol-gels

Gels

4 Citations (Scopus)

Evaluating the performance of a hybrid artificial liver support system with a recoverable hepatic failure rat model

Ijima, H., Noguchi, A., Katsuno, T., Ono, T., Nakazawa, K., Funatsu, K. & Kawakami, K., 2001, In : Annals of the New York Academy of Sciences. 944, p. 344-349 6 p.

Research output: Contribution to journal › Article

Artificial Liver

Liver Failure

Liver

Rats

Warm Ischemia

14 Citations (Scopus)

Newly developed aminopropyl-silicate immunoisolation membrane for a microcapsule-shaped bioartificial pancreas

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., 2001, In : Annals of the New York Academy of Sciences. 944, p. 277-283 7 p.

Research output: Contribution to journal › Article

Silicates

Capsules

Pancreas

Membranes

Gels

Selective extraction of active α-chymotrypsin by reversed micelles

Goto, M., Korenaga, M., Ono, T. & Furusaki, S., 2001, In : Kagaku Kogaku Ronbunshu. 27, 2, p. 184-185 2 p.

Research output: Contribution to journal › Article

Chymotrypsin

Micelles

Phosphoric acid

Proteins

Anionic surfactants

80 Citations (Scopus)

Synthesis and transport characterization of alginate/aminopropyl-silicate/alginate microcapsule: Application to bioartificial pancreas

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., 2001, In : Biomaterials. 22, 21, p. 2827-2834 8 p.

Research output: Contribution to journal › Article

Silicates

Alginate

Capsules

Pancreas

Gels

2000

4 Citations (Scopus)

Catalytic capabilities of lipase immobilized into organically modified silicates by sol-gel method

Furukawa, S. Y., Ono, T., Ijima, H. & Kawakami, K., 2000, In : Kagaku Kogaku Ronbunshu. 26, 6, p. 885-886 2 p.

Research output: Contribution to journal › Article

Silicates

Lipases

Lipase

Sol-gel process

Diatomaceous Earth

45 Citations (Scopus)

Control of molecular weight cut-off immunoisolation by multilayering glycol chitosan-alginate polyion complex on alginate-based microcapsules

Sakai, S., Ono, T., Ijima, H. & Kawakami, K., 2000, In : Journal of Microencapsulation. 17, 6, p. 691-699 9 p.

Research output: Contribution to journal › Article

Alginate

Glycols

Chitosan

globulins

Capsules

21 Citations (Scopus)

Important parameters affecting efficiency of protein refolding by reversed micelles

Goto, M., Hashimoto, Y., Fujita, T. A., Ono, T. & Furusaki, S., 2000, In : Biotechnology Progress. 16, 6, p. 1079-1085 7 p.

Research output: Contribution to journal › Article

Protein Refolding

Micelles

micelles

Pancreatic Ribonuclease

Proteins

1999

31 Citations (Scopus)

Extraction of DNA by reversed micelles

Goto, M., Ono, T., Horiuchi, A. & Furusaki, S., 1999, In : Journal of Chemical Engineering of Japan. 32, 1, p. 123-125 3 p.

Research output: Contribution to journal › Article

Micelles

DNA

Cationic surfactants

Deoxyribonucleotides

Coulomb interactions

Reversed micelles as novel protein refolding media

Goto, M., Ono, T. & Furusaki, S., 1999, In : ACS Symposium Series. 740, p. 374-383 10 p.

Research output: Contribution to journal › Article

Micelles

Pancreatic Ribonuclease

Proteins

Liquids

Surface-Active Agents

1998

11 Citations (Scopus)

Catalytic properties of novel reversed micellar system on trans-esterification by α-chymotrypsin in organic media

Iskandar, L., Ono, T., Kamiya, N., Goto, M., Nakashio, F. & Furusaki, S., Sep 1998, In : Biochemical Engineering Journal. 2, 1, p. 29-33 5 p.

Research output: Contribution to journal › Article

Esterification

Transesterification

Chymotrypsin

Micelles

Sodium

32 Citations (Scopus)

Extraction and activity of chymotrypsin using AOT-DOLPA mixed reversed micellar systems

Goto, M., Ishikawa, Y., Ono, T., Nakashio, F. & Alan Hatton, T., Sep 1998, In : Biotechnology Progress. 14, 5, p. 729-734 6 p.

Research output: Contribution to journal › Article

phosphoric acid

Chymotrypsin

Micelles

chymotrypsin

micelles

8 Citations (Scopus)

Factors affecting protein transfer into surfactant-isooctane solution: A case study of extraction behavior of chemically modified cytochrome c

Ono, T. & Goto, M., 1998, In : Biotechnology Progress. 14, 6, p. 903-908 6 p.

Research output: Contribution to journal › Article

cytochrome c

Cytochromes c

Surface-Active Agents

surfactants

case studies

48 Citations (Scopus)

Protein refolding by reversed micelles utilizing solid-liquid extraction technique

Hashimoto, Y., Ono, T., Goto, M. & Hatton, T. A., Mar 5 1998, In : Biotechnology and Bioengineering. 57, 5, p. 620-623 4 p.

Research output: Contribution to journal › Article

Protein Refolding

Micelles

Pancreatic Ribonuclease

Proteins

Liquids

1997

60 Citations (Scopus)

Design of surfactants suitable for protein extraction by reversed micelles

Goto, M., Ono, T., Nakashio, F. & Hatton, T. A., Apr 5 1997, In : Biotechnology and Bioengineering. 54, 1, p. 26-32 7 p.

Research output: Contribution to journal › Article

Micelles

Surface-Active Agents

Surface active agents

Proteins

Heptanes

1996

7 Citations (Scopus)

Effect of organic solvents on extraction behaviour of cytochrome c using reversed micellar solutions

Ono, T., Miyazaki, T., Goto, M. & Nakashio, F., 1996, In : Solvent Extraction Research and Development. 1996, 3, p. 1-9 9 p.

Research output: Contribution to journal › Article

Cytochromes c

Organic solvents

Proteins

Micelles

Hexanes

48 Citations (Scopus)

Extraction behavior of hemoglobin using reversed micelles by dioleyl phosphoric acid

Ono, T., Goto, M., Nakashio, F. & Hatton, T. A., Nov 1996, In : Biotechnology Progress. 12, 6, p. 793-800 8 p.

Research output: Contribution to journal › Article

phosphoric acid

Micelles

micelles

hemoglobin

Hemoglobins

2 Citations (Scopus)

Novel immobilized enzymes utilizing reversed micellar solutions as an optical resolution tool

Ono, T., Goto, M., Nakashio, F. & Hatton, T. A., Aug 1996, In : Journal of Chemical Engineering of Japan. 29, 4, p. 695-701 7 p.

Research output: Contribution to journal › Article

Microemulsions

Enzymes

3 Citations (Scopus)

Reversed micelles recognize an active protein

Goto, M., Ono, T., Nakashio, F. & Hatton, T. A., 1996, In : Biotechnology Techniques. 10, 3, p. 141-144 4 p.

Research output: Contribution to journal › Article

Chymotrypsin

Micelles

Proteins

Phosphoric acid

1995

10 Citations (Scopus)

Protein extraction by new reversed micelles with di(tridecyl) phosphoric acid

Goto, M., Kuroki, M., Ono, T. & Nakashio, F., Jan 1995, In : Separation Science and Technology. 30, 1, p. 89-99 11 p.

Research output: Contribution to journal › Article

Micelles

Phosphoric acid

Proteins

Surface-Active Agents

Surface active agents