If you made any changes in Pure these will be visible here soon.

Research Output 2003 2019

  • 543 Citations
  • 13 h-Index
  • 24 Article
  • 1 Review article
2019

Differential scanning fluorimetric analysis of the amino-acid binding to taste receptor using a model receptor protein, the ligand-binding domain of fish T1r2a/T1r3

Yoshida, T., Yasui, N., Kusakabe, Y., Ito, C., Akamatsu, M. & Yamashita, A., Jan 1 2019, In : PloS one. 14, 10, e0218909.

Research output: Contribution to journalArticle

Open Access
fluorometry
Protein Binding
Fish
Fishes
Fluorometry

Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli

Nakatani, T., Yasui, N., Tamura, I. & Yamashita, A., Mar 18 2019, In : Scientific reports. 9, 1, p. 4722 4722.

Research output: Contribution to journalArticle

Open Access
Green Fluorescent Proteins
Lysine
Escherichia coli
Fluorescence
Biological Science Disciplines
2017

Functional analyses of specific protein-protein interaction networks using affinity clamping technology

Yasui, N., Jan 1 2017, In : Seikagaku. 89, 2, p. 293-297 5 p.

Research output: Contribution to journalReview article

Protein Interaction Maps
Constriction
Technology
Proteins

Immunoaffinity purification of the glycosylated extracellular fragment of mouse Plexin A2 produced in a mammalian expression system

Nogi, T., Mihara, E., Yasui, N. & Takagi, J., 2017, In : Methods in Molecular Biology. 1493, p. 57-72 16 p.

Research output: Contribution to journalArticle

Semaphorins
varespladib methyl
Glycosylation
Disulfides
Signal Transduction
8 Citations (Scopus)

Structural basis for ligand capture and release by the endocytic receptor ApoER2

Hirai, H., Yasui, N., Yamashita, K., Tabata, S., Yamamoto, M., Takagi, J. & Nogi, T., 2017, (Accepted/In press) In : EMBO Reports.

Research output: Contribution to journalArticle

Ligands
Conformations
LDL Receptors
Endocytosis
Signal transduction
25 Citations (Scopus)

Structural basis for perception of diverse chemical substances by T1r taste receptors

Nuemket, N., Yasui, N., Kusakabe, Y., Nomura, Y., Atsumi, N., Akiyama, S., Nango, E., Kato, Y., Kaneko, M. K., Takagi, J., Hosotani, M. & Yamashita, A., May 23 2017, In : Nature Communications. 8, 15530.

Research output: Contribution to journalArticle

taste
Taste Perception
Amino Acids
Ligands
Sugar Acids
2016
12 Citations (Scopus)

Taste substance binding elicits conformational change of taste receptor T1r heterodimer extracellular domains

Nango, E., Akiyama, S., Maki-Yonekura, S., Ashikawa, Y., Kusakabe, Y., Krayukhina, E., Maruno, T., Uchiyama, S., Nuemket, N., Yonekura, K., Shimizu, M., Atsumi, N., Yasui, N., Hikima, T., Yamamoto, M., Kobayashi, Y. & Yamashita, A., May 10 2016, In : Scientific Reports. 6, 25745.

Research output: Contribution to journalArticle

Ligands
Taste Perception
Energy Transfer
Dimerization
G-Protein-Coupled Receptors
2014
22 Citations (Scopus)

Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation

Yasui, N., Findlay, G. M., Gish, G. D., Hsiung, M. S., Huang, J., Tucholska, M., Taylor, L., Smith, L., Boldridge, W. C., Koide, A., Pawson, T. & Koide, S., Jun 19 2014, In : Molecular Cell. 54, 6, p. 1034-1041 8 p.

Research output: Contribution to journalArticle

Protein Interaction Maps
Phosphotyrosine
Embryonic Stem Cells
Cell Differentiation
Endoderm
2013
41 Citations (Scopus)

Dissection of the BCR-ABL signaling network using highly specific monobody inhibitors to the SHP2 SH2 domains

Sha, F., Gencer, E. B., Georgeon, S., Koide, A., Yasui, N., Koide, S. & Hantschel, O., Sep 10 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 37, p. 14924-14929 6 p.

Research output: Contribution to journalArticle

SH2 Domain-Containing Protein Tyrosine Phosphatases
src Homology Domains
Dissection
Phosphoric Monoester Hydrolases
Phosphotyrosine
2012
37 Citations (Scopus)

Broad ranges of affinity and specificity of anti-histone antibodies revealed by a quantitative peptide immunoprecipitation assay

Nishikori, S., Hattori, T., Fuchs, S. M., Yasui, N., Wojcik, J., Koide, A., Strahl, B. D. & Koide, S., Dec 14 2012, In : Journal of Molecular Biology. 424, 5, p. 391-399 9 p.

Research output: Contribution to journalArticle

Immunoprecipitation
Histone Code
Histones
Chromatin Immunoprecipitation
Anti-Idiotypic Antibodies
2011
5 Citations (Scopus)

Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain

Nakata, Z., Nagae, M., Yasui, N., Bujo, H., Nogi, T. & Takagi, J., Jan 2011, In : Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 67, 1, p. 129-132 4 p.

Research output: Contribution to journalArticle

Protein Transport
Crystallization
classifying
Sorting
crystallization
16 Citations (Scopus)

Functional importance of covalent homodimer of reelin protein linked via its central region

Yasui, N., Kitago, Y., Beppu, A., Kohno, T., Morishita, S., Gomi, H., Nagae, M., Hattori, M. & Takagi, J., Oct 7 2011, In : Journal of Biological Chemistry. 286, 40, p. 35247-35256 10 p.

Research output: Contribution to journalArticle

Cysteine
Disulfides
Neurons
Assays
Oligomerization
15 Citations (Scopus)

Structural basis for variant-specific neuroligin-binding by α-neurexin

Tanaka, H., Nogi, T., Yasui, N., Iwasaki, K. & Takagi, J., 2011, In : PLoS One. 6, 4, e19411.

Research output: Contribution to journalArticle

Sex Hormone-Binding Globulin
Structural Models
Laminin
Epidermal Growth Factor
Cell Adhesion
2010
6 Citations (Scopus)

Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation

Yasui, N., Mihara, E., Nampo, M., Tamura-Kawakami, K., Unno, H., Matsumoto, K. & Takagi, J., Jan 31 2010, In : Journal of Immunological Methods. 352, 1-2, p. 153-160 8 p.

Research output: Contribution to journalArticle

Monoclonal Antibodies
Wnt Signaling Pathway
Fluorescence Microscopy
Immunoprecipitation
Detergents
104 Citations (Scopus)

Structural basis for semaphorin signalling through the plexin receptor

Nogi, T., Yasui, N., Mihara, E., Matsunaga, Y., Noda, M., Yamashita, N., Toyofuku, T., Uchiyama, S., Goshima, Y., Kumanogoh, A. & Takagi, J., Oct 28 2010, In : Nature. 467, 7319, p. 1123-1127 5 p.

Research output: Contribution to journalArticle

Semaphorins
varespladib methyl
GTPase-Activating Proteins
Ligands
Membranes
43 Citations (Scopus)

Structural Basis for Specific Recognition of Reelin by Its Receptors

Yasui, N., Nogi, T. & Takagi, J., Mar 10 2010, In : Structure. 18, 3, p. 320-331 12 p.

Research output: Contribution to journalArticle

LDL Receptors
Mutagenesis
Glycoproteins
Proteins
Ligands
2008
18 Citations (Scopus)

Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification

Nogi, T., Sangawa, T., Tabata, S., Nagae, M., Tamura-Kawakami, K., Beppu, A., Hattori, M., Yasui, N. & Takagi, J., Dec 2008, In : Protein Science. 17, 12, p. 2120-2126 7 p.

Research output: Contribution to journalArticle

Purification
Peptides
Antibodies
Proteins
Organic solvents
8 Citations (Scopus)

Structure of the F-spondin reeler domain reveals a unique β-sandwich fold with a deformable disulfide-bonded loop

Nagae, M., Nishikawa, K., Yasui, N., Yamasaki, M., Nogi, T. & Takagi, J., Oct 18 2008, In : Acta Crystallographica Section D: Biological Crystallography. 64, 11, p. 1138-1145 8 p.

Research output: Contribution to journalArticle

Thrombospondin 1
Extracellular Matrix Proteins
Disulfides
Immunoglobulins
Glycoproteins
2007
49 Citations (Scopus)

Structure of a receptor-binding fragment of reelin and mutational analysis reveal a recognition mechanism similar to endocytic receptors

Yasui, N., Nogi, T., Kitao, T., Nakano, Y., Hattori, M. & Takagi, J., Jun 12 2007, In : Proceedings of the National Academy of Sciences of the United States of America. 104, 24, p. 9988-9993 6 p.

Research output: Contribution to journalArticle

LDL Receptors
Lipoprotein Receptors
Mutagenesis
Alanine
Binding Sites
2006
27 Citations (Scopus)

Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography

Nogi, T., Yasui, N., Hattori, M., Iwasaki, K. & Takagi, J., Aug 9 2006, In : EMBO Journal. 25, 15, p. 3675-3683 9 p.

Research output: Contribution to journalArticle

X Ray Tomography
Electron Microscope Tomography
X ray crystallography
X Ray Crystallography
Epidermal Growth Factor
2005
39 Citations (Scopus)

Involvement of the collagen I-binding motif in the anti-angiogenic activity of pigment epithelium-derived factor

Hosomichi, J., Yasui, N., Koide, T., Soma, K. & Morita, I., Sep 30 2005, In : Biochemical and Biophysical Research Communications. 335, 3, p. 756-761 6 p.

Research output: Contribution to journalArticle

Collagen
Tumors
Growth
Neoplasms
pigment epithelium-derived factor
2003
13 Citations (Scopus)

Collagen-protein interactions mapped by phototriggered thiol introduction.

Yasui, N., 2003, In : J Am Chem Soc.. 125, 51, p. 15728-15728 1 p.

Research output: Contribution to journalArticle

55 Citations (Scopus)

Dual-site recognition of different extracellular matrix components by anti-angiogenic/neurotrophic serpin, PEDF

Yasui, N., Mori, T., Morito, D., Matsushita, O., Kourai, H., Nagata, K. & Koide, T., Mar 25 2003, In : Biochemistry. 42, 11, p. 3160-3167 8 p.

Research output: Contribution to journalArticle

Serine Proteinase Inhibitors
Extracellular Matrix
Collagen
Binding Sites
Heparin